Molecular cloning - Page 7

High-level gene expression in Lactobacillus plantarum using a pheromone-regulated bacteriocin promoter

Pheromones / Gene expression / Multidisciplinary / Applied Economics Letters / Bacteriocins / Escherichia coli / Lactobacillus / Glucuronidase / Molecular cloning / Lactobacillus Plantarum / Lactococcus lactis / Escherichia coli / Lactobacillus / Glucuronidase / Molecular cloning / Lactobacillus Plantarum / Lactococcus lactis

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Biophysical characterization of a recombinant leucyl aminopeptidase from Bacillus kaustophilus

Biochemistry / Thermodynamics / Dentistry / Structural Biology / Molecular Dynamics Simulation / Genomics / Protein Folding / Proteomics / Biological Physics / Fluorescent Dyes and Reagents / Apoptosis / Structural Genomics / Ion Channels / Macromolecular X-Ray Crystallography / Protein Stability / HIV / Neurospora crassa / Cell line / Humans / Sequence alignment / Computer Simulation / Circular Dichroism / Mice / Animals / Bacillus / Ligand Binding / Temperature / Urea / Enzyme / Molecular cloning / Amino Acids / Protein Sequence Analysis / Cell nucleus / Anti-Bacterial Agents / Protein Secondary Structure Prediction / Success Rate / Spectrum analysis / Trifluoroethanol / Transfection / Protein Conformation / Molecular weight / Large Scale / Amino Acid Sequence / Calcium Chloride / Protein Quaternary Structure / Recombinant Protein / Structure activity Relationship / Recombinant Proteins / Protein Binding / Hydrogen-Ion Concentration / X Ray Crystallography / Structure Determination / Protein Denaturation / Ligands / Sodium Chloride / Biochemistry and cell biology / Solvents / Genomics / Protein Folding / Proteomics / Biological Physics / Fluorescent Dyes and Reagents / Apoptosis / Structural Genomics / Ion Channels / Macromolecular X-Ray Crystallography / Protein Stability / HIV / Neurospora crassa / Cell line / Humans / Sequence alignment / Computer Simulation / Circular Dichroism / Mice / Animals / Bacillus / Ligand Binding / Temperature / Urea / Enzyme / Molecular cloning / Amino Acids / Protein Sequence Analysis / Cell nucleus / Anti-Bacterial Agents / Protein Secondary Structure Prediction / Success Rate / Spectrum analysis / Trifluoroethanol / Transfection / Protein Conformation / Molecular weight / Large Scale / Amino Acid Sequence / Calcium Chloride / Protein Quaternary Structure / Recombinant Protein / Structure activity Relationship / Recombinant Proteins / Protein Binding / Hydrogen-Ion Concentration / X Ray Crystallography / Structure Determination / Protein Denaturation / Ligands / Sodium Chloride / Biochemistry and cell biology / Solvents

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Thioredoxin peroxidases of the malarial parasite Plasmodium falciparum

Kinetics / Antioxidants / Peroxidase / Sequence alignment / Animals / Hydrogen Peroxide / Molecular cloning / Thioredoxin / Peroxidases / Substrate Specificity / Plasmodium falciparum / Cysteine / Molecular weight / Amino Acid Sequence / Recombinant Proteins / Biochemistry and cell biology / Peroxiredoxins / Hydrogen Peroxide / Molecular cloning / Thioredoxin / Peroxidases / Substrate Specificity / Plasmodium falciparum / Cysteine / Molecular weight / Amino Acid Sequence / Recombinant Proteins / Biochemistry and cell biology / Peroxiredoxins

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Characterization of a plant-like protochlorophyllide a divinyl reductase in green sulfur bacteria

Biological Chemistry / Biological Sciences / Phylogeny / Polymerase Chain Reaction / Rhodobacter / CHEMICAL SCIENCES / Molecular cloning / Base Sequence / Recombinant Proteins / PLANT PROTEINS / Chlorobium / Green Sulfur Bacteria / CHEMICAL SCIENCES / Molecular cloning / Base Sequence / Recombinant Proteins / PLANT PROTEINS / Chlorobium / Green Sulfur Bacteria

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Identification and characterization of endoglucanases for fungicidal activity in Anabaena laxa (Cyanobacteria)

Electrophysiology / Immune response / Kinetics / MicroRNA / Mass Spectrometry / Flow Cytometry / Calcium / Biological Chemistry / Fluorescent Dyes and Reagents / RNA silencing / Dendritic Cells / Signal Transduction / Polyamines / Biological Sciences / RNA interference / Dogs / Plant Genome Project / Cell line / Environmental Sciences / Humans / Sequence alignment / Protein Kinases / Applied / Applied Phycology / Animals / Monoclonal Antibodies / Oryza Sativa / Peptides / Dendritic cell / Chemotaxis / Nucleic Acids / Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis / Small RNA / Mitogen Activated Protein Kinase / CHEMICAL SCIENCES / Molecular cloning / Rats / Experimental Medicine / Genomic Library / Amino Acid Profile / SIGNAL PEPTIDE / Amino Acid Sequence / Benzoxazoles / Cell Wall / Open Reading Frame / Recombinant Proteins / Protein Binding / Bacterial Infection / Ligands / Cyclic nucleotide / Protein Kinase Inhibitors / cDNA library / Nucleotides / Gene Expression Regulation / Adenosine Triphosphate / Intracellular Calcium / Tissue Specificity / Host Defense / Flow Cytometry / Calcium / Biological Chemistry / Fluorescent Dyes and Reagents / RNA silencing / Dendritic Cells / Signal Transduction / Polyamines / Biological Sciences / RNA interference / Dogs / Plant Genome Project / Cell line / Environmental Sciences / Humans / Sequence alignment / Protein Kinases / Applied / Applied Phycology / Animals / Monoclonal Antibodies / Oryza Sativa / Peptides / Dendritic cell / Chemotaxis / Nucleic Acids / Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis / Small RNA / Mitogen Activated Protein Kinase / CHEMICAL SCIENCES / Molecular cloning / Rats / Experimental Medicine / Genomic Library / Amino Acid Profile / SIGNAL PEPTIDE / Amino Acid Sequence / Benzoxazoles / Cell Wall / Open Reading Frame / Recombinant Proteins / Protein Binding / Bacterial Infection / Ligands / Cyclic nucleotide / Protein Kinase Inhibitors / cDNA library / Nucleotides / Gene Expression Regulation / Adenosine Triphosphate / Intracellular Calcium / Tissue Specificity / Host Defense

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RETRACTED: Antiviral and antiparasite properties of an l-amino acid oxidase from the Snake Bothrops jararaca: Cloning and identification of a complete cDNA sequence

Leishmania / Dengue Virus / Cell line / Animals / Biochemical / Molecular cloning / Trypanosoma Cruzi / Bothrops / Amino Acid Profile / BIOCHEMICAL PHARMACOLOGY / Amino Acid Sequence / Cell Survival / Aedes / Antiviral Agents / Molecular cloning / Trypanosoma Cruzi / Bothrops / Amino Acid Profile / BIOCHEMICAL PHARMACOLOGY / Amino Acid Sequence / Cell Survival / Aedes / Antiviral Agents

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Description of an elasmobranch TCR coreceptor: CD8α from Rhinobatos productus

Immunology / Phylogeny / Sequence alignment / Animals / Elasmobranchii / Developmental / Molecular cloning / Protein Sequence Analysis / Amino Acid Profile / T cell activation / SIGNAL PEPTIDE / Amino Acid Sequence / Open Reading Frame / Full Length Movies / Developmental / Molecular cloning / Protein Sequence Analysis / Amino Acid Profile / T cell activation / SIGNAL PEPTIDE / Amino Acid Sequence / Open Reading Frame / Full Length Movies

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P2Y6 receptor mediates colonic NaCl secretion via differential activation of cAMP-mediated transport

Immunohistochemistry / Xenopus / Video microscopy / cyclic AMP / Animals / Molecular cloning / Rats / Colon / Oocytes / Clinical Investigation / Sodium Chloride / Adenosine Triphosphate / Molecular cloning / Rats / Colon / Oocytes / Clinical Investigation / Sodium Chloride / Adenosine Triphosphate

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Recombinant expression, activity screening and functional characterization identifies three novel endo-1,4-β-glucanases that efficiently hydrolyse cellulosic substrates

Applied microbiology / Gene expression / Multidisciplinary / Cellulose / Cellulase / Aspergillus niger / Enzyme / Applied Microbiology and Biotechnology / Molecular cloning / Heterologous Expression / Recombinant Proteins / Ascomycota / Production Cost / BASIDIOMYCOTA / Aspergillus niger / Enzyme / Applied Microbiology and Biotechnology / Molecular cloning / Heterologous Expression / Recombinant Proteins / Ascomycota / Production Cost / BASIDIOMYCOTA

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Phylogenetic analyses of DNA sequences reveal species partitions amongst isolates of< i> Armillaria</i> from Africa

Microbiology / Africa / Molecular Evolution / Plant Biology / Phylogeny / Sequence alignment / Polymerase Chain Reaction / Molecular cloning / Genetic variation / Base Sequence / DNA sequence / Sequence alignment / Polymerase Chain Reaction / Molecular cloning / Genetic variation / Base Sequence / DNA sequence

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Identification and partial characterization of extracellular aspartic protease genes from Metschnikowia pulcherrima IWBT Y1123 and Candida apicola IWBT Y1384

Engineering / Biochemistry / Microbiology / Parasitology / Physiology / Zoology / Oncology / Technology / Developmental Biology / Immunology / Kinetics / Biotechnology / Plant Biology / Medical Microbiology / Membrane Biology / Membrane Proteins / Cell Biology / Centromere Biology / Gene expression / Multidisciplinary / Sequence Analysis / Signal Transduction / Biological Sciences / Mitosis / Cercopithecus aethiops / Cell line / Humans / Natural Killer cells / Human nature / Neospora / Mice / Animals / Applied Environmental Microbiology / Polymerase Chain Reaction / Cell Fractionation / Biochemical / Proteins / Plasmids / Soil sciences / Plant Physiology and Biochemistry / Clinical Sciences / CHEMICAL SCIENCES / Cytokine / Molecular cloning / Kinesin / Fluorescent Antibody Technique / Protein Secondary Structure Prediction / Substrate Specificity / Tumor necrosis factor-alpha / Ticks and Tick Borne Diseases / Veterinary Sciences / Molecular weight / Protozoan Proteins / Amino Acid Sequence / Base Sequence / Human Fibroblasts / Subcellular Fractions / Ribonucleotides / DNA binding proteins / Vero cells / Biochemistry and cell biology / Reading Frames / Interleukin / Neurochemical / Zoology / Oncology / Technology / Developmental Biology / Immunology / Kinetics / Biotechnology / Plant Biology / Medical Microbiology / Membrane Biology / Membrane Proteins / Cell Biology / Centromere Biology / Gene expression / Multidisciplinary / Sequence Analysis / Signal Transduction / Biological Sciences / Mitosis / Cercopithecus aethiops / Cell line / Humans / Natural Killer cells / Human nature / Neospora / Mice / Animals / Applied Environmental Microbiology / Polymerase Chain Reaction / Cell Fractionation / Biochemical / Proteins / Plasmids / Soil sciences / Plant Physiology and Biochemistry / Clinical Sciences / CHEMICAL SCIENCES / Cytokine / Molecular cloning / Kinesin / Fluorescent Antibody Technique / Protein Secondary Structure Prediction / Substrate Specificity / Tumor necrosis factor-alpha / Ticks and Tick Borne Diseases / Veterinary Sciences / Molecular weight / Protozoan Proteins / Amino Acid Sequence / Base Sequence / Human Fibroblasts / Subcellular Fractions / Ribonucleotides / DNA binding proteins / Vero cells / Biochemistry and cell biology / Reading Frames / Interleukin / Neurochemical

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High-density mutagenesis by combined DNA shuffling and phage display to assign essential amino acid residues in protein-protein interactions: application to study structure-function of plasminogen activation inhibitor 1 (PAI-I)

Molecular Biology / Recombinant DNA Technology / Protein-Protein Interaction / Humans / Mutagenesis / Sequence alignment / Mice / Animals / Epitope mapping / Phage display / Surface plasmon resonance / Monoclonal Antibodies / Shell Half-Life / Molecular cloning / Surface Area / Amino Acid Profile / High Density Concrete / Monoclonal Antibody / Tissue Plasminogen Activator / Amino Acid Substitution Rates / Structure activity Relationship / Protein Binding / D structure / Structure Function / Epitopes / Plasminogen Activator Inhibitor / Biochemistry and cell biology / Sequence alignment / Mice / Animals / Epitope mapping / Phage display / Surface plasmon resonance / Monoclonal Antibodies / Shell Half-Life / Molecular cloning / Surface Area / Amino Acid Profile / High Density Concrete / Monoclonal Antibody / Tissue Plasminogen Activator / Amino Acid Substitution Rates / Structure activity Relationship / Protein Binding / D structure / Structure Function / Epitopes / Plasminogen Activator Inhibitor / Biochemistry and cell biology

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Functional characterization of a stilbene synthase gene using a transient expression system in planta

Functional Analysis / Plant Biology / Mass Spectrometry / Tobacco / Cell Culture / Real Time / Enzyme / Molecular cloning / Western blot / Stilbenes / Arachis Hypogaea / PLANT PROTEINS / Transgenic plant / Transient Expression / Real Time / Enzyme / Molecular cloning / Western blot / Stilbenes / Arachis Hypogaea / PLANT PROTEINS / Transgenic plant / Transient Expression

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Two clones obtained from Urabe AM9 mumps virus vaccine differ in their replicative efficiency in neuroblastoma cells

Microbiology / Immunology / Kinetics / Medical Microbiology / Real-time RT-PCR / Cercopithecus aethiops / Cell line / Brain / Humans / Mumps / Microbes / Animals / Neuroblastoma / Molecular cloning / Mumps Virus / Rats / Nervous System / Vero cells / Cercopithecus aethiops / Cell line / Brain / Humans / Mumps / Microbes / Animals / Neuroblastoma / Molecular cloning / Mumps Virus / Rats / Nervous System / Vero cells

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Expression of a synthetic gene encoding a Tribolium castaneum carboxylesterase in Pichia pastoris

Escherichia coli / Protein Secretion / Animals / Pichia pastoris / Molecular cloning / Protein expression and purification / Tribolium / Specific Activity / Base Sequence / Heterologous Expression / Tribolium Castaneum / Biochemistry and cell biology / Protein expression and purification / Tribolium / Specific Activity / Base Sequence / Heterologous Expression / Tribolium Castaneum / Biochemistry and cell biology

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Expression of Polymorphic msp1beta Genes during Acute Anaplasma marginale Rickettsemia

Polymorphism / Gene expression / Biological Sciences / Infection and immunity / Antibodies / Animals / Infection / Cattle / Molecular cloning / Amino Acids / Anaplasma / Bacteremia / B Lymphocytes / Amino Acid Sequence / Epitopes / Merozoite surface protein 1 / Animals / Infection / Cattle / Molecular cloning / Amino Acids / Anaplasma / Bacteremia / B Lymphocytes / Amino Acid Sequence / Epitopes / Merozoite surface protein 1

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