Molecular cloning

Cyanide detoxification by recombinant bacterial rhodanese

Genetics / Catalysis / Water Purification / Multidisciplinary / Escherichia coli / Genetically modified organisms / Detoxification / Pseudomonas aeruginosa / Enzyme / Molecular cloning / Natural Environment / Western blot / Protein Expression / Pseudomonas Aeruginosa / Cyanides / Recombinant Proteins / Cellular system / Genetically modified organisms / Detoxification / Pseudomonas aeruginosa / Enzyme / Molecular cloning / Natural Environment / Western blot / Protein Expression / Pseudomonas Aeruginosa / Cyanides / Recombinant Proteins / Cellular system

Production and purification of a recombinant elastomeric polypeptide, G-(VPGVG)19-VPGV, from Escherichia coli

Engineering / Technology / Biotechnology / Magnetic Resonance Spectroscopy / Biological Sciences / Escherichia coli / Polymerase Chain Reaction / Peptides / Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis / Molecular cloning / Amino Acid Sequence / Base Sequence / Escherichia coli / Polymerase Chain Reaction / Peptides / Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis / Molecular cloning / Amino Acid Sequence / Base Sequence

First Complete Amino Acid Sequence of a Polar Tube Protein in a Microporidian Species, Encephalitozoon cuniculi

Microbiology / Zoology / Animals / Polymerase Chain Reaction / Gene Dosage / Molecular cloning / Molecular weight / Protozoan Proteins / Eukaryotic microbiology / Amino Acid Sequence / Isoelectric point / Molecular cloning / Molecular weight / Protozoan Proteins / Eukaryotic microbiology / Amino Acid Sequence / Isoelectric point

Morphogenesis of Maize Embryos Requires ZmPRPL35-1 Encoding a Plastid Ribosomal Protein

Electron Microscopy / Pattern Formation / Confocal Microscopy / Morphogenesis / In Situ Hybridization / Biological Sciences / Plant Physiology / Mutation / Maize / Plant / Ribosomal proteins / Phenotype / Molecular cloning / Zea mays / Seeds / Amino Acid Sequence / Embryos / PLANT PROTEINS / Plant Epidermis / Biological Sciences / Plant Physiology / Mutation / Maize / Plant / Ribosomal proteins / Phenotype / Molecular cloning / Zea mays / Seeds / Amino Acid Sequence / Embryos / PLANT PROTEINS / Plant Epidermis

The Hansenula polymorpha PEX14 gene encodes a novel peroxisomal membrane protein essential for peroxisome biogenesis

Membrane Proteins / Western blotting / Gene Prediction / Biological Sciences / Catalase / Membrane transport proteins / Molecular cloning / Amino Acid Sequence / Membrane Protein / Electron Microscope / Membrane transport proteins / Molecular cloning / Amino Acid Sequence / Membrane Protein / Electron Microscope

Southern molecular hybridization experiments with parallel complementary DNA probes

Drosophila melanogaster / DNA / Animals / Nucleic acid hybridization / Molecular cloning / Base Sequence / Biochemistry and cell biology / Base Sequence / Biochemistry and cell biology

Two clones obtained from Urabe AM9 mumps virus vaccine differ in their replicative efficiency in neuroblastoma cells

Microbiology / Immunology / Kinetics / Medical Microbiology / Real-time RT-PCR / Cercopithecus aethiops / Cell line / Brain / Humans / Mumps / Microbes / Animals / Neuroblastoma / Molecular cloning / Mumps Virus / Rats / Nervous System / Vero cells / Cercopithecus aethiops / Cell line / Brain / Humans / Mumps / Microbes / Animals / Neuroblastoma / Molecular cloning / Mumps Virus / Rats / Nervous System / Vero cells

Luminal Heterodimeric Amino Acid Transporter Defective in Cystinuria

Molecular Biology / In Situ Hybridization / Biological Sciences / Humans / Sequence alignment / Kidney / Mice / Animals / Male / Molecular cloning / Amino Acids / Fluorescent Antibody Technique / Xenopus laevis / Amino Acid Sequence / Oocytes / Kidney / Mice / Animals / Male / Molecular cloning / Amino Acids / Fluorescent Antibody Technique / Xenopus laevis / Amino Acid Sequence / Oocytes

AtPUMP: an Arabidopsis gene encoding a plant uncoupling mitochondrial protein

Membrane Proteins / Mitochondria / Ion Channels / Potato / Arabidopsis thaliana / Gene Dosage / Arabidopsis / Molecular cloning / Amino Acid Profile / Molecular weight / Energy Transfer / Amino Acid Sequence / Base Sequence / Open Reading Frame / Cold Acclimation / Uncoupling Protein / PLANT PROTEINS / Nucleotides / Biochemistry and cell biology / Gene Dosage / Arabidopsis / Molecular cloning / Amino Acid Profile / Molecular weight / Energy Transfer / Amino Acid Sequence / Base Sequence / Open Reading Frame / Cold Acclimation / Uncoupling Protein / PLANT PROTEINS / Nucleotides / Biochemistry and cell biology

Expression of a mouse brain cDNA encoding novel gamma-aminobutyric acid transporter

Kinetics / Membrane Proteins / Gene expression / Biological Sciences / DNA / Xenopus / Brain / Kidney / Liver / Mice / Animals / Biological / Membrane transport proteins / CHEMICAL SCIENCES / Molecular cloning / Amino Acid Sequence / Base Sequence / Oocytes / Gamma-Aminobutyric Acid / Betaine / Xenopus / Brain / Kidney / Liver / Mice / Animals / Biological / Membrane transport proteins / CHEMICAL SCIENCES / Molecular cloning / Amino Acid Sequence / Base Sequence / Oocytes / Gamma-Aminobutyric Acid / Betaine

Identification and Characterization of  gamma-Glutamylamine Cyclotransferase, an Enzyme Responsible for  gamma-Glutamyl-epsilon-lysine Catabolism

Catalysis / Biological Chemistry / Macromolecular X-Ray Crystallography / Biological Sciences / Humans / CHEMICAL SCIENCES / Molecular cloning / Protein Conformation / Amino Acid Sequence / CHEMICAL SCIENCES / Molecular cloning / Protein Conformation / Amino Acid Sequence

Cryptosporidium parvum: Functional Complementation of a Parasite Transcriptional Coactivator CpMBF1 in Yeast

Genetics / Microbiology / Transcription Factors / Saccharomyces cerevisiae / Experimental parasitology / Cell Differentiation / Sequence alignment / Animals / Polymerase Chain Reaction / Enzyme / Molecular cloning / DNA binding / Veterinary Sciences / Genome sequence / Amino Acid Sequence / Open Reading Frame / Cell Differentiation / Sequence alignment / Animals / Polymerase Chain Reaction / Enzyme / Molecular cloning / DNA binding / Veterinary Sciences / Genome sequence / Amino Acid Sequence / Open Reading Frame

Arabidopsis Chloroplastic Glutaredoxin C5 as a Model to Explore Molecular Determinants for Iron-Sulfur Cluster Binding into Glutaredoxins

Biological Chemistry / Macromolecular X-Ray Crystallography / Biological Sciences / Biological / Arabidopsis / CHEMICAL SCIENCES / Molecular cloning / Liquid Chromatography / Electrospray Ionization Mass Spectrometry / Subcellular Fractions / Protein Binding / Chloroplasts / CHEMICAL SCIENCES / Molecular cloning / Liquid Chromatography / Electrospray Ionization Mass Spectrometry / Subcellular Fractions / Protein Binding / Chloroplasts

Human placental syncytiotrophoblastic Mr 75,000 polypeptide defined by antibodies to a synthetic peptide based on a cloned human endogenous retroviral DNA sequence

Multidisciplinary / Antibodies / Cell line / Pregnancy / Humans / Placenta / Female / DNA sequence design / Molecular cloning / Oncogenes / Molecular weight / Epitopes / Placenta / Female / DNA sequence design / Molecular cloning / Oncogenes / Molecular weight / Epitopes

Egfl7, a novel epidermal growth factor-domain gene expressed in endothelial cells

Aging / Gene expression / Biological Sciences / Cell line / Humans / Sequence alignment / Endothelial Cells / Mice / Animals / Endoplasmic Reticulum / Proteins / Developmental / Molecular cloning / Epidermal Growth Factor / Embryonic Development / Endothelial cell / Amino Acid Sequence / Golgi Apparatus / Gene Expression Regulation / Gene expression profiling / Sequence alignment / Endothelial Cells / Mice / Animals / Endoplasmic Reticulum / Proteins / Developmental / Molecular cloning / Epidermal Growth Factor / Embryonic Development / Endothelial cell / Amino Acid Sequence / Golgi Apparatus / Gene Expression Regulation / Gene expression profiling
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