Molecular cloning - Page 5

Efficient double stranded sequencing of cDNA clones containing long poly(A) tails using anchored poly(dT) primers

Biological Sciences / DNA / Environmental Sciences / Nucleic Acids / Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis / Molecular cloning / Poly A / Molecular cloning / Poly A

Download

Dengue-1 envelope protein domain III produced in Pichia pastoris: Potential use for serological diagnosis

Dengue Virus / Humans / Dengue / Expression / Molecular cloning / Protein expression and purification / Amino Acid Sequence / Base Sequence / Recombinant Proteins / Enzyme Linked Immunosorbent Assay / Biochemistry and cell biology / immunoglobulin G / Protein expression and purification / Amino Acid Sequence / Base Sequence / Recombinant Proteins / Enzyme Linked Immunosorbent Assay / Biochemistry and cell biology / immunoglobulin G

Download

Gene rescue in plants by direct gene transfer of total genomic DNA into protoplasts

Tobacco / Biological Sciences / Environmental Sciences / Drug Resistance / Genome / Nucleic Acids / Plasmids / Molecular cloning / Genomic DNA / Protoplasts / Transfection / Gene Transfer / Nucleic Acids / Plasmids / Molecular cloning / Genomic DNA / Protoplasts / Transfection / Gene Transfer

Download

Some Rare Indo-Pacific Coral Species Are Probable Hybrids

Gene Flow / Population Genetics / Multidisciplinary / Genetic Diversity / Phylogeny / Anthozoa / Haplotypes / Animals / Polymerase Chain Reaction / Coral Reef / Population Size / Effective Population Size / PLoS one / Introns / Molecular cloning / Genetic variation / Oceans and Seas / Anthozoa / Haplotypes / Animals / Polymerase Chain Reaction / Coral Reef / Population Size / Effective Population Size / PLoS one / Introns / Molecular cloning / Genetic variation / Oceans and Seas

Download

Identification and intracellular location of MAGE-3 gene product

Cancer / Immunohistochemistry / Gene expression / Cell line / Mice / Melanoma / Animals / Monoclonal Antibodies / Nickel / Molecular cloning / Monoclonal Antibody / Base Sequence / Fusion Protein / Melanoma / Animals / Monoclonal Antibodies / Nickel / Molecular cloning / Monoclonal Antibody / Base Sequence / Fusion Protein

Download

Identification and Intracellular Location of MAGE3 Gene Product1

Cancer / Immunohistochemistry / Gene expression / Cell line / Mice / Melanoma / Animals / Monoclonal Antibodies / Nickel / Molecular cloning / Monoclonal Antibody / Base Sequence / Fusion Protein / Melanoma / Animals / Monoclonal Antibodies / Nickel / Molecular cloning / Monoclonal Antibody / Base Sequence / Fusion Protein

Download

A novel plant peptidyl-prolyl-cis-trans-isomerase (PPIase): cDNA cloning, structural analysis, enzymatic activity and expression

Genetics / Plant Biology / Enzyme Inhibitors / Plant Molecular Biology / Tacrolimus / Escherichia coli / Structure Analysis / Molecular cloning / Seeds / Calmodulin / Amino Acid Profile / Heat Shock Proteins / Amino Acid Sequence / Open Reading Frame / Triticum / Gene Family / Protein Binding / Embryos / Enzymatic Activity / Sirolimus / DNA binding proteins / Binding Site / Biochemistry and cell biology / Escherichia coli / Structure Analysis / Molecular cloning / Seeds / Calmodulin / Amino Acid Profile / Heat Shock Proteins / Amino Acid Sequence / Open Reading Frame / Triticum / Gene Family / Protein Binding / Embryos / Enzymatic Activity / Sirolimus / DNA binding proteins / Binding Site / Biochemistry and cell biology

Download

Method Enabling Fast Partial Sequencing of cDNA Clones

Analytical Chemistry / Humans / Expressed Sequence Tags / Analytical / Similarity Search / Enzyme / Molecular cloning / Time Factors / Very high throughput / Analytical Biochemistry / Base Sequence / cDNA library / Nucleotides / Biochemistry and cell biology / Dna Synthesis / DNA sequence / Enzyme / Molecular cloning / Time Factors / Very high throughput / Analytical Biochemistry / Base Sequence / cDNA library / Nucleotides / Biochemistry and cell biology / Dna Synthesis / DNA sequence

Download

Barramundi (Lates calcarifer) desaturase with Δ6/Δ8 dual activities

Engineering / Technology / Australia / Gene expression / Biological Sciences / Saccharomyces cerevisiae / Liver / Animals / Fishes / Molecular cloning / Alpha-linolenic acid / Saccharomyces cerevisiae / Liver / Animals / Fishes / Molecular cloning / Alpha-linolenic acid

Download

Biochemical Characterization of an L-Xylulose Reductase from Neurospora crassa

Kinetics / Gene expression / Multidisciplinary / Neurospora crassa / Glucose Metabolism / Xylitol / Escherichia coli / Applied Environmental Microbiology / Temperature / Enzyme / Molecular cloning / Substrate Specificity / Guinea Pig / Xylose Reductase / Heterologous Expression / Recombinant Proteins / Hydrogen-Ion Concentration / Pigeon / Pentose Phosphate Pathway / Xylitol / Escherichia coli / Applied Environmental Microbiology / Temperature / Enzyme / Molecular cloning / Substrate Specificity / Guinea Pig / Xylose Reductase / Heterologous Expression / Recombinant Proteins / Hydrogen-Ion Concentration / Pigeon / Pentose Phosphate Pathway

Download

Genetic Characterization of Human Immunodeficiency Virus Type 1 in Elite Controllers: Lack of Gross Genetic Defects or Common Amino Acid Changes

Genetics / Microbiology / Immunology / Polymorphism / Molecular Evolution / Medical Microbiology / Virology / Mozambique / Adolescent / HIV / Genetic Diversity / Biological Sciences / Phylogeny / Pregnancy / Humans / Child / Sequence alignment / Mutation / Microbes / Female / Blood / Drug Resistance / Blood sampling / Male / Sexual Behavior / Polymerase Chain Reaction / Infant / AIDS / Clinical Sciences / Vagina / Middle Aged / Molecular cloning / Human immunodeficiency virus / Adult / Time Factors / Highly Active Antiretroviral Therapy / Hiv seropositivity / Amino Acid Profile / Semen / Amino Acid Sequence / Base Sequence / Lymphocytes / Reverse Transcriptase / Binding Site / Viral Load / Medical Microbiology / Virology / Mozambique / Adolescent / HIV / Genetic Diversity / Biological Sciences / Phylogeny / Pregnancy / Humans / Child / Sequence alignment / Mutation / Microbes / Female / Blood / Drug Resistance / Blood sampling / Male / Sexual Behavior / Polymerase Chain Reaction / Infant / AIDS / Clinical Sciences / Vagina / Middle Aged / Molecular cloning / Human immunodeficiency virus / Adult / Time Factors / Highly Active Antiretroviral Therapy / Hiv seropositivity / Amino Acid Profile / Semen / Amino Acid Sequence / Base Sequence / Lymphocytes / Reverse Transcriptase / Binding Site / Viral Load

Download

An ABC transporter encoding gene lndW confers resistance to landomycin E

Microbiology / Medical Microbiology / Gene expression / Streptomyces / Aminoglycosides / Plasmids / Molecular cloning / Anti-Bacterial Agents / Microbial Sensitivity Tests / Open Reading Frame / Protein Binding / DNA fragmentation / Plasmids / Molecular cloning / Anti-Bacterial Agents / Microbial Sensitivity Tests / Open Reading Frame / Protein Binding / DNA fragmentation

Download

The rug3 Locus of Pea Encodes Plastidial Phosphoglucomutase

Biological Sciences / Plant Physiology / Plant / Genetic linkage analysis / Enzyme / Molecular cloning / Amino Acid Sequence / Base Sequence / Molecular cloning / Amino Acid Sequence / Base Sequence

Download

Hypothermia modulates circadian clock gene expression in lizard peripheral tissues

Physiology / RNA / Gene expression / Biological Sciences / Hypothermia / Humans / Models / Circadian Rhythm / Lizards / Animals / Light / Temperature / American / Gene / Molecular cloning / Gen / Species Specificity / Cryptochromes / Cryptochrome / Circadian Clock / Period / Humans / Models / Circadian Rhythm / Lizards / Animals / Light / Temperature / American / Gene / Molecular cloning / Gen / Species Specificity / Cryptochromes / Cryptochrome / Circadian Clock / Period

Download

Resolution, configurational assignment, and enantiopharmacology of 2-amino-3-[3-hydroxy-5-(2-methyl-2H-tetrazol-5-yl)isoxazol-4-yl]propionic acid, a potent GluR3- and GluR4-preferring AMPA receptor agonist

Analytical Chemistry / Organic Chemistry / Electrophysiology / Macromolecular X-Ray Crystallography / Chirality / Glutamate receptors / Female / Animals / Propionic Acid / Resolution / High Pressure Liquid Chromatography / Molecular cloning / Rats / Radioligand Assay / Xenopus laevis / Protein Conformation / Chiral HPLC / Alanine / Oocytes / Structure activity Relationship / Recombinant Proteins / X Ray Crystallography / Molecular Structure / Glutamate Receptor / Glutamate receptors / Female / Animals / Propionic Acid / Resolution / High Pressure Liquid Chromatography / Molecular cloning / Rats / Radioligand Assay / Xenopus laevis / Protein Conformation / Chiral HPLC / Alanine / Oocytes / Structure activity Relationship / Recombinant Proteins / X Ray Crystallography / Molecular Structure / Glutamate Receptor

Download

Ultra-Deep Pyrosequencing (UDPS) Data Treatment to Study Amplicon HCV Minor Variants

Multidisciplinary / Humans / Mutation / Polymerase Chain Reaction / PLoS one / Molecular cloning / Reproducibility of Results / Genetic variation / Molecular cloning / Reproducibility of Results / Genetic variation

Download