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Molecular cloning

Comparative biochemical characterization of three exolytic oligoalginate lyases from Vibrio splendidus reveals complementary substrate scope, temperature, and pH adaptations

Multidisciplinary / Applied Environmental Microbiology / Temperature / Molecular cloning / Vibrio / Alginates / Substrate Specificity / Molecular weight / Culture Media / Hydrogen-Ion Concentration / D-glucuronic acid / Alginates / Substrate Specificity / Molecular weight / Culture Media / Hydrogen-Ion Concentration / D-glucuronic acid

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Assignment of Disulphide Bridges in Par j 2.0101, a Major Allergen of Parietaria judaica Pollen

Biological Chemistry / Humans / Pollen / Escherichia coli / Allergens / Immunoglobulin E / Molecular cloning / Amino Acid Sequence / Recombinant Proteins / Biochemistry and cell biology / Molecular Sequence Data / Immunoglobulin E / Molecular cloning / Amino Acid Sequence / Recombinant Proteins / Biochemistry and cell biology / Molecular Sequence Data

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Characterization of a chaperone ClpB homologue of Paracoccidioides brasiliensis

Microbiology / Industrial Biotechnology / Humans / Sequence alignment / Yeast / Molecular cloning / Heat Shock Proteins / Amino Acid Sequence / Base Sequence / Paracoccidioides / Molecular Sequence Data / Molecular cloning / Heat Shock Proteins / Amino Acid Sequence / Base Sequence / Paracoccidioides / Molecular Sequence Data

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Cloning of a Candida albicans peptide transport gene

Microbiology / Kinetics / Multidisciplinary / Saccharomyces cerevisiae / Candida albicans / Peptides / Membrane transport proteins / Molecular cloning / Amino Acids / Genomic Library / Amino Acid Sequence / Base Sequence / Molecular Sequence Data / Peptides / Membrane transport proteins / Molecular cloning / Amino Acids / Genomic Library / Amino Acid Sequence / Base Sequence / Molecular Sequence Data

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Cloning and sequencing of two Candida parapsilosis genes encoding acid proteases

General / General Microbiology / Candida / Nitrogen / Molecular cloning / Genomic Library / Amino Acid Profile / Amino Acid Sequence / Base Sequence / Bovine Serum Albumin / DNA sequence / Molecular Sequence Data / Genomic Library / Amino Acid Profile / Amino Acid Sequence / Base Sequence / Bovine Serum Albumin / DNA sequence / Molecular Sequence Data

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Monofunctional catalase P ofParacoccidioides brasiliensis: identification, characterization, molecular cloning and expression analysis

Microbiology / Industrial Biotechnology / Phylogeny / Sequence alignment / Polymerase Chain Reaction / Catalase / Yeast / Hydrogen Peroxide / Molecular cloning / Molecular weight / Amino Acid Sequence / Base Sequence / Expression analysis / Paracoccidioides / Isoelectric point / Molecular Sequence Data / Catalase / Yeast / Hydrogen Peroxide / Molecular cloning / Molecular weight / Amino Acid Sequence / Base Sequence / Expression analysis / Paracoccidioides / Isoelectric point / Molecular Sequence Data

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Phylogenetic identification of Sparganum proliferum as a pseudophyllidean cestode

Microbiology / Parasitology / Medical Microbiology / PCR / Phylogeny / mtDNA / Mitochondrial DNA / Human Body / Animals / Polymerase Chain Reaction / Cytochrome c oxidase / Life Cycle / Molecular cloning / Cestoda / Veterinary Sciences / Amino Acid Sequence / Base Sequence / Succinate Dehydrogenase / DNA sequence / Molecular Sequence Data / mtDNA / Mitochondrial DNA / Human Body / Animals / Polymerase Chain Reaction / Cytochrome c oxidase / Life Cycle / Molecular cloning / Cestoda / Veterinary Sciences / Amino Acid Sequence / Base Sequence / Succinate Dehydrogenase / DNA sequence / Molecular Sequence Data

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Homodimeric β-galactosidase from Lactobacillus delbrueckii subsp. bulgaricus DSM 20081: expression in Lactobacillus plantarum and biochemical characterization

Engineering / Gene expression / Agricultural / CHEMICAL SCIENCES / Molecular cloning / Lactobacillus Plantarum / Agricultural and Food Chemistry / Lactose / Recombinant Proteins / Lactobacillus Plantarum / Agricultural and Food Chemistry / Lactose / Recombinant Proteins

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Identification, characterization and phylogenetic signal of an elongation factor-1 alpha fragment in demosponges (Metazoa, Porifera, Demospongiae)

Evolutionary Biology / Zoology / Molecular Systematics / Molecular Evolution / Phylogeny / Animals / Gene Order / Molecular cloning / Molecular Marker / Larva / Moths / Biochemistry and molecular biology / Molecular weight / Amino Acid Sequence / Base Sequence / Phylogenetic Reconstruction / Biochemistry and cell biology / Molecular Sequence Data / Animals / Gene Order / Molecular cloning / Molecular Marker / Larva / Moths / Biochemistry and molecular biology / Molecular weight / Amino Acid Sequence / Base Sequence / Phylogenetic Reconstruction / Biochemistry and cell biology / Molecular Sequence Data

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Functional Characterization and Expression of a Cytosolic Iron-Superoxide Dismutase from Cowpea Root Nodules

Physiology / Electrophysiology / British / Immunohistochemistry / Mitochondria / Gene expression / Western blotting / Free Radical / Biological Sciences / Phylogeny / Plant Physiology / Sequence alignment / Escherichia coli / Nitric oxide / Animals / Plant / Male / Vascular endothelium / American / Cyclic peptides / Iron / Medical Physiology / Soybean / Plant Roots / Superoxide Dismutase / Enzyme / Subcellular Localization / Molecular cloning / Rats / Fabaceae / Phaseolus vulgaris / Radioligand Assay / Endothelin-1 / Potassium Channels / Wistar Rats / Common bean / Amino Acid Profile / Amino Acid Sequence / Glycine max / Carotid Artery / Recombinant Proteins / Vasodilation / Ligands / Catalytic Activity / Vigna Unguiculata / PISUM SATIVUM / Piperidines / Chlorides / Endothelins / Molecular Sequence Data / Gene expression / Western blotting / Free Radical / Biological Sciences / Phylogeny / Plant Physiology / Sequence alignment / Escherichia coli / Nitric oxide / Animals / Plant / Male / Vascular endothelium / American / Cyclic peptides / Iron / Medical Physiology / Soybean / Plant Roots / Superoxide Dismutase / Enzyme / Subcellular Localization / Molecular cloning / Rats / Fabaceae / Phaseolus vulgaris / Radioligand Assay / Endothelin-1 / Potassium Channels / Wistar Rats / Common bean / Amino Acid Profile / Amino Acid Sequence / Glycine max / Carotid Artery / Recombinant Proteins / Vasodilation / Ligands / Catalytic Activity / Vigna Unguiculata / PISUM SATIVUM / Piperidines / Chlorides / Endothelins / Molecular Sequence Data

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Characterization of a gene which encodes a mannosyltransferase homolog of Paracoccidioides brasiliensis

Microbiology / Immunology / Medical Microbiology / Humans / Microbes / Escherichia coli / Polymerase Chain Reaction / Molecular cloning / Amino Acid Profile / Molecular Mass / Amino Acid Sequence / Base Sequence / Open Reading Frame / Membrane Protein / Recombinant Protein / Recombinant Proteins / Paracoccidioides / Molecular Sequence Data / Escherichia coli / Polymerase Chain Reaction / Molecular cloning / Amino Acid Profile / Molecular Mass / Amino Acid Sequence / Base Sequence / Open Reading Frame / Membrane Protein / Recombinant Protein / Recombinant Proteins / Paracoccidioides / Molecular Sequence Data

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Intramembrane proteolytic cleavage by human signal peptide peptidase like 3 and malaria signal peptide peptidase

Physiology / Membrane Proteins / Enzyme Inhibitors / Humans / Animals / Cell Death / Hepatitis C Virus / Medical Physiology / Molecular cloning / Active site / Plasmodium falciparum / Protozoan Proteins / MHC class I / Biochemistry and cell biology / Proteolytic activity / Cell Death / Hepatitis C Virus / Medical Physiology / Molecular cloning / Active site / Plasmodium falciparum / Protozoan Proteins / MHC class I / Biochemistry and cell biology / Proteolytic activity

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Catalytic antisense RNAs produced by incorporating ribozyme cassettes into cDNA

Genetics / Gene / Plant viruses / Molecular cloning / Base Sequence / Molecular Sequence Data

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The Larger Genomic RNA of Helicoverpa armigera Stunt Tetravirus Encodes the Viral RNA Polymerase and Has a Novel 3′-Terminal tRNA-like Structure

Virology / RNA polymerase / Lepidoptera / Biological Sciences / Insect Viruses / Sequence alignment / Animals / Molecular cloning / Host Range / Hepatitis E Virus / Amino Acid Sequence / RNA Dependent RNA Polymerase / RNA Virus / Nucleotides / Helicoverpa armigera / Molecular Sequence Data / Sequence alignment / Animals / Molecular cloning / Host Range / Hepatitis E Virus / Amino Acid Sequence / RNA Dependent RNA Polymerase / RNA Virus / Nucleotides / Helicoverpa armigera / Molecular Sequence Data

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Nueva estrategia para la construcción de una genoteca genómica de Pinus pinaster en cromosomas artificiales de bacterias

Molecular cloning / Genome Organization / Genomic Library / Picea abies / Pinus Pinaster

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Assignment of Disulphide Bridges in Par j 2.0101, a Major Allergen of Parietaria judaica Pollen

Biological Chemistry / Humans / Pollen / Escherichia coli / Allergens / Immunoglobulin E / Molecular cloning / Amino Acid Sequence / Recombinant Proteins / Biochemistry and cell biology / Immunoglobulin E / Molecular cloning / Amino Acid Sequence / Recombinant Proteins / Biochemistry and cell biology

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