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Molecular cloning
Molecular cloning
A polyketide synthase acyltransferase domain structure suggests a recognition mechanism for its hydroxymalonyl-acyl carrier protein substrate
Engineering / Physics / Chemistry / Biology / Medicine / Multidisciplinary / Protein Engineering / Macromolecular X-Ray Crystallography / Peptides / PLoS one / Molecular cloning / Substrate Specificity / Multienzyme complexes / Bacillus Cereus / Multidisciplinary / Protein Engineering / Macromolecular X-Ray Crystallography / Peptides / PLoS one / Molecular cloning / Substrate Specificity / Multienzyme complexes / Bacillus Cereus
Biochemical and genetic characterization of Trypanosoma cruzi N -myristoyltransferase
Kinetics / Biological Sciences / Cercopithecus aethiops / Animals / Genetically modified organisms / Biochemical / CHEMICAL SCIENCES / Molecular cloning / Trypanosoma Cruzi / Recombinant Proteins / Vero cells / Biochemical / CHEMICAL SCIENCES / Molecular cloning / Trypanosoma Cruzi / Recombinant Proteins / Vero cells
BYC, an atypical aspartic endopeptidase from Rhipicephalus (Boophilus) microplus eggs
Physiology / Immune response / Catalysis / Mass Spectrometry / Fluorescence / Catalytic Mechanism / Humans / Female / Animals / Peptides / D-Aspartic Acid / High Performance Liquid Chromatography / Cattle / Molecular cloning / Protein Sequence Analysis / Specific Activity / Substrate Specificity / Protein Conformation / Amino Acid Sequence / Recombinant Proteins / Rhipicephalus (Boophilus) microplus / Catalytic Activity / Biochemistry and cell biology / Rhipicephalus / Catalytic Mechanism / Humans / Female / Animals / Peptides / D-Aspartic Acid / High Performance Liquid Chromatography / Cattle / Molecular cloning / Protein Sequence Analysis / Specific Activity / Substrate Specificity / Protein Conformation / Amino Acid Sequence / Recombinant Proteins / Rhipicephalus (Boophilus) microplus / Catalytic Activity / Biochemistry and cell biology / Rhipicephalus
Expression, Purification, and Partial Characterization of HCV RNA Polymerase
RNA polymerase / Biological Sciences / Escherichia coli / Glutathione / Hepatitis C Virus / Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis / Inclusion Bodies / Molecular cloning / Molecular weight / Enzymatic Activity / Fusion Protein / Biochemistry and cell biology / Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis / Inclusion Bodies / Molecular cloning / Molecular weight / Enzymatic Activity / Fusion Protein / Biochemistry and cell biology
Heterodimer formation is essential for heparanase enzymatic activity
Extracellular Matrix / Western blotting / Cell line / Humans / Processing / Peptides / Glucuronidase / Biochemical / Plasmids / Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis / Molecular cloning / Amino Acids / Amino Acid Profile / Transfection / Glutathione Transferase / Enzymatic Activity / Biochemistry and cell biology / Dimerization / Immunoblotting / Peptides / Glucuronidase / Biochemical / Plasmids / Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis / Molecular cloning / Amino Acids / Amino Acid Profile / Transfection / Glutathione Transferase / Enzymatic Activity / Biochemistry and cell biology / Dimerization / Immunoblotting
An Unstable Trinucleotide-Repeat Region on Chromosome 13 Implicated in Spinocerebellar Ataxia: A Common Expansion Locus
Christianity / Schizophrenia / Bipolar Disorder / Borderline Personality Disorder / Biological Sciences / Humans / Genetic Testing / Female / Spinocerebellar ataxia / Male / Depressive Disorder / Pedigree / Genetic linkage analysis / Molecular cloning / Adult / Reproducibility of Results / Trinucleotide Repeat Disorders / The American / Humans / Genetic Testing / Female / Spinocerebellar ataxia / Male / Depressive Disorder / Pedigree / Genetic linkage analysis / Molecular cloning / Adult / Reproducibility of Results / Trinucleotide Repeat Disorders / The American
Identification and characterization of endoglucanases for fungicidal activity in Anabaena laxa (Cyanobacteria)
Electrophysiology / Immune response / Kinetics / MicroRNA / Mass Spectrometry / Flow Cytometry / Calcium / Biological Chemistry / Fluorescent Dyes and Reagents / RNA silencing / Dendritic Cells / Signal Transduction / Polyamines / Biological Sciences / RNA interference / Dogs / Plant Genome Project / Cell line / Environmental Sciences / Humans / Sequence alignment / Protein Kinases / Applied / Applied Phycology / Animals / Monoclonal Antibodies / Oryza Sativa / Peptides / Dendritic cell / Chemotaxis / Nucleic Acids / Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis / Small RNA / Mitogen Activated Protein Kinase / CHEMICAL SCIENCES / Molecular cloning / Rats / Experimental Medicine / Genomic Library / Amino Acid Profile / SIGNAL PEPTIDE / Amino Acid Sequence / Benzoxazoles / Cell Wall / Open Reading Frame / Recombinant Proteins / Protein Binding / Bacterial Infection / Ligands / Cyclic nucleotide / Protein Kinase Inhibitors / cDNA library / Nucleotides / Gene Expression Regulation / Adenosine Triphosphate / Intracellular Calcium / Tissue Specificity / Host Defense / Flow Cytometry / Calcium / Biological Chemistry / Fluorescent Dyes and Reagents / RNA silencing / Dendritic Cells / Signal Transduction / Polyamines / Biological Sciences / RNA interference / Dogs / Plant Genome Project / Cell line / Environmental Sciences / Humans / Sequence alignment / Protein Kinases / Applied / Applied Phycology / Animals / Monoclonal Antibodies / Oryza Sativa / Peptides / Dendritic cell / Chemotaxis / Nucleic Acids / Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis / Small RNA / Mitogen Activated Protein Kinase / CHEMICAL SCIENCES / Molecular cloning / Rats / Experimental Medicine / Genomic Library / Amino Acid Profile / SIGNAL PEPTIDE / Amino Acid Sequence / Benzoxazoles / Cell Wall / Open Reading Frame / Recombinant Proteins / Protein Binding / Bacterial Infection / Ligands / Cyclic nucleotide / Protein Kinase Inhibitors / cDNA library / Nucleotides / Gene Expression Regulation / Adenosine Triphosphate / Intracellular Calcium / Tissue Specificity / Host Defense
Transforming a fructan:fructan 6G-fructosyltransferase from perennial ryegrass into a sucrose:sucrose 1-fructosyltransferase
Kinetics / Biological Sciences / Plant Physiology / Sequence alignment / Plant / Molecular cloning / Substrate Specificity / Amino Acid Sequence / PLANT PROTEINS / Site-directed Mutagenesis / Molecular cloning / Substrate Specificity / Amino Acid Sequence / PLANT PROTEINS / Site-directed Mutagenesis
In vivo protein transduction: biologically active intact pep-1-superoxide dismutase fusion protein efficiently protects against ischemic insult
Genetics / Neuronal cell death / Free Radical / Hippocampus / Reactive Oxygen Species / Mice / Animals / Male / Peptides / Protein structure / Brain Ischemia / Neurons / Astrocytes / Epidermis / Superoxide Dismutase / Antioxidant enzyme / Molecular cloning / Amino Acid Profile / Biological activity / Reperfusion injury / Human Disease / Fusion Protein / Full Length Movies / Biochemistry and cell biology / Mice / Animals / Male / Peptides / Protein structure / Brain Ischemia / Neurons / Astrocytes / Epidermis / Superoxide Dismutase / Antioxidant enzyme / Molecular cloning / Amino Acid Profile / Biological activity / Reperfusion injury / Human Disease / Fusion Protein / Full Length Movies / Biochemistry and cell biology
Xyn10A, a Thermostable Endoxylanase from Acidothermus cellulolyticus 11B
Multidisciplinary / Applied / Applied Environmental Microbiology / Shell Half-Life / Molecular cloning / Actinomycetales / Xylans / Hydrogen-Ion Concentration / *Hot Temperature / Actinomycetales / Xylans / Hydrogen-Ion Concentration / *Hot Temperature
A lepidopteran pacifastin member: Cloning, gene structure, recombinant production, transcript profiling and in vitro activity
Zoology / Enzyme Inhibitors / Fungi / Gene expression / Sequence alignment / Escherichia coli / Animals / Proteins / Gene Structure / Enzyme / Molecular cloning / Insect Biochemistry and Molecular Biology / Amino Acid Sequence / Molecular Identification / Recombinant Proteins / Entomopathogenic Fungi / Insect Biochemistry / Biochemistry and cell biology / Bombyx mori / Escherichia coli / Animals / Proteins / Gene Structure / Enzyme / Molecular cloning / Insect Biochemistry and Molecular Biology / Amino Acid Sequence / Molecular Identification / Recombinant Proteins / Entomopathogenic Fungi / Insect Biochemistry / Biochemistry and cell biology / Bombyx mori
A gene encoding a Superoxide dismutase of the facultative intracellular bacterium Listeria monocytogenes
Genetics / Manganese / Escherichia coli / Nucleic acid hybridization / Listeria monocytogenes / Page / Gene / Bp / Superoxide Dismutase / Molecular cloning / Amino Acid Sequence / Base Sequence / Kb / R / L / Page / Gene / Bp / Superoxide Dismutase / Molecular cloning / Amino Acid Sequence / Base Sequence / Kb / R / L
An ABC transporter encoding gene lndW confers resistance to landomycin E
Microbiology / Medical Microbiology / Gene expression / Streptomyces / Aminoglycosides / Plasmids / Molecular cloning / Anti-Bacterial Agents / Microbial Sensitivity Tests / Open Reading Frame / Protein Binding / DNA fragmentation / Plasmids / Molecular cloning / Anti-Bacterial Agents / Microbial Sensitivity Tests / Open Reading Frame / Protein Binding / DNA fragmentation
Homodimeric β-Galactosidase from Lactobacillus delbrueckii subsp. bulgaricus DSM 20081: Expression in Lactobacillus plantarum and Biochemical Characterization
Engineering / Gene expression / Agricultural / CHEMICAL SCIENCES / Molecular cloning / Lactobacillus Plantarum / Agricultural and Food Chemistry / Lactose / Recombinant Proteins / Lactobacillus Plantarum / Agricultural and Food Chemistry / Lactose / Recombinant Proteins
Constitutive expression of Botrytis aclada laccase in Pichia pastoris
Kinetics / Gene expression / Genetic Engineering / Botrytis / Laccase / Fermentation / Molecular cloning / Bioreactors / Bioengineered / Fermentation / Molecular cloning / Bioreactors / Bioengineered
Modification of human beta-globin locus PAC clones by homologous recombination in Escherichia coli
Genetics / Biological Sciences / High Frequency / Environmental Sciences / Humans / Escherichia coli / Nucleic Acids / Plasmids / Molecular cloning / Genetic Recombination / Homologous Recombination / Genome sequence / Recombinant Protein / Recombinant Proteins / Genetic Markers / Escherichia coli / Nucleic Acids / Plasmids / Molecular cloning / Genetic Recombination / Homologous Recombination / Genome sequence / Recombinant Protein / Recombinant Proteins / Genetic Markers
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