Molecular cloning

Dengue-1 envelope protein domain III produced in Pichia pastoris: Potential use for serological diagnosis

Dengue Virus / Humans / Dengue / Molecular cloning / Protein expression and purification / Amino Acid Sequence / Base Sequence / Recombinant Proteins / Enzyme Linked Immunosorbent Assay / Biochemistry and cell biology / immunoglobulin G / Amino Acid Sequence / Base Sequence / Recombinant Proteins / Enzyme Linked Immunosorbent Assay / Biochemistry and cell biology / immunoglobulin G

Inter-specific sequence conservation and intra-individual sequence variation in a spider silk gene

Spiders / Silk / Animals / Biological / Molecular cloning / Genomic DNA / Genetic variation / Amino Acid Profile / Amino Acid Sequence / Biological Macromolecules / Biochemistry and cell biology / Genomic DNA / Genetic variation / Amino Acid Profile / Amino Acid Sequence / Biological Macromolecules / Biochemistry and cell biology

P450arom gene expression in peripheral blood lymphocytes: Identification of a cryptic splice site for exon-1 afterEpstein–Barr virus transformation

Gene expression / Humans / Female / Male / Polymerase Chain Reaction / Alternative splicing / Genetic transformation / Peripheral blood lymphocyte / Molecular cloning / Epstein-Barr virus / Alternative Splicing / Base Sequence / Lymphocytes / Biochemistry and cell biology / Tissue Specificity / Aromatase Activity / Ribonucleases / Androstenedione / Alternative splicing / Genetic transformation / Peripheral blood lymphocyte / Molecular cloning / Epstein-Barr virus / Alternative Splicing / Base Sequence / Lymphocytes / Biochemistry and cell biology / Tissue Specificity / Aromatase Activity / Ribonucleases / Androstenedione

Hypothermia modulates circadian clock gene expression in lizard peripheral tissues

Physiology / RNA / Gene expression / Biological Sciences / Hypothermia / Humans / Models / Circadian Rhythm / Lizards / Animals / Light / Temperature / American / Gene / Molecular cloning / Gen / Species Specificity / Cryptochromes / Cryptochrome / Circadian Clock / Period / Humans / Models / Circadian Rhythm / Lizards / Animals / Light / Temperature / American / Gene / Molecular cloning / Gen / Species Specificity / Cryptochromes / Cryptochrome / Circadian Clock / Period

Hypothermia modulates circadian clock gene expression in lizard peripheral tissues

Physiology / RNA / Gene expression / Biological Sciences / Hypothermia / Humans / Models / Circadian Rhythm / Lizards / Animals / Light / Temperature / American / Gene / Molecular cloning / Gen / Species Specificity / Cryptochromes / Cryptochrome / Circadian Clock / Period / Humans / Models / Circadian Rhythm / Lizards / Animals / Light / Temperature / American / Gene / Molecular cloning / Gen / Species Specificity / Cryptochromes / Cryptochrome / Circadian Clock / Period

RecA-Assisted Rapid Enrichment of Specific Clones from Model DNA Libraries

Cellulose / Recombinant DNA Technology / Humans / Biochemical / Affinity chromatography / Molecular cloning / DNA probes / Biochemistry and cell biology / Molecular cloning / DNA probes / Biochemistry and cell biology

MultiBac complexomics

Macromolecular X-Ray Crystallography / Western blotting / Genetic Engineering / Escherichia coli / Animals / Plasmids / Glycoproteins / Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis / Molecular cloning / Recombinant Proteins / Biochemistry and cell biology / Plasmids / Glycoproteins / Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis / Molecular cloning / Recombinant Proteins / Biochemistry and cell biology

Nucleotide sequence and expression of a novel pectate lyase gene (pel-3) and a closely linked endopolygalacturonase gene (peh-1) of Erwinia carotovora subsp. carotovora 71

Regulation / Gene expression / Multidisciplinary / Structural Analysis / Sequence alignment / Escherichia coli / Applied Environmental Microbiology / Bacteria / Homology / Applied and Environmental Microbiology / Genetic linkage analysis / Molecular cloning / Species Specificity / Amino Acid Sequence / Base Sequence / Polygalacturonase / Escherichia coli / Applied Environmental Microbiology / Bacteria / Homology / Applied and Environmental Microbiology / Genetic linkage analysis / Molecular cloning / Species Specificity / Amino Acid Sequence / Base Sequence / Polygalacturonase

Two clones obtained from Urabe AM9 mumps virus vaccine differ in their replicative efficiency in neuroblastoma cells

Microbiology / Immunology / Kinetics / Medical Microbiology / Real-time RT-PCR / Cercopithecus aethiops / Cell line / Brain / Humans / Mumps / Microbes / Animals / Neuroblastoma / Molecular cloning / Mumps Virus / Rats / Nervous System / Vero cells / Cercopithecus aethiops / Cell line / Brain / Humans / Mumps / Microbes / Animals / Neuroblastoma / Molecular cloning / Mumps Virus / Rats / Nervous System / Vero cells

A reorganized Candida albicans DNA sequence promoting homologous non-integrative genetic transformation

DNA replication / Molecular Microbiology / Biological Sciences / Saccharomyces cerevisiae / Candida albicans / Genetic transformation / Plasmids / Molecular cloning / Base Sequence / DNA sequence / Genetic transformation / Plasmids / Molecular cloning / Base Sequence / DNA sequence

PapD, a periplasmic transport protein in P-pilus biogenesis

Bacteriology / Biological Sciences / Escherichia coli / Molecular cloning / Methionine / Molecular weight / Amino Acid Sequence / Base Sequence / Bacterial Adhesion / Molecular weight / Amino Acid Sequence / Base Sequence / Bacterial Adhesion

Vector design for optimal protein expression

Technology / Gene expression / Biological Sciences / Animals / Proteins / Molecular cloning / Xenopus laevis / Transfection / Codon / Ribosomes / Poly A / Drug Stability / Protein Biosynthesis / Molecular cloning / Xenopus laevis / Transfection / Codon / Ribosomes / Poly A / Drug Stability / Protein Biosynthesis

Survivin protein expression in bovine follicular oocytes and their in vitro developmental competence

Immunohistochemistry / Western blotting / Biological Sciences / Pregnancy / Escherichia coli / Female / Animals / Blastocyst / Caspase / Cattle / Molecular cloning / mRna expression levels / Embryonic Development / In vitro culture / Protein Expression / Oocytes / Recombinant Proteins / Embryos / Female / Animals / Blastocyst / Caspase / Cattle / Molecular cloning / mRna expression levels / Embryonic Development / In vitro culture / Protein Expression / Oocytes / Recombinant Proteins / Embryos

Extracellular Proteases of <i>Trichoderma</i> Species

Biotechnology / Medical Microbiology / Plants / Trichoderma / Molecular cloning / Molecular weight / Isoelectric point / Molecular weight / Isoelectric point

Production of soluble, active acetyl serotonin methyl transferase in Leishmania tarentolae

Leishmania / Gene expression / Serotonin / Humans / Escherichia coli / Solubility / Enzyme / Molecular cloning / Protein expression and purification / Protein Expression / Recombinant Proteins / Biochemistry and cell biology / Solubility / Enzyme / Molecular cloning / Protein expression and purification / Protein Expression / Recombinant Proteins / Biochemistry and cell biology

Mammalian Mitochondrial DNA Sequences Can Function as in Vivo Bacterial Transcription Terminators

Mitochondrial DNA / Escherichia coli / Female / Animals / Plasmids / Molecular cloning / Rats / Base Sequence / Nucleic Acid Conformation / Biochemistry and cell biology / Molecular cloning / Rats / Base Sequence / Nucleic Acid Conformation / Biochemistry and cell biology

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