Molecular cloning

Cloning and nucleotide sequence of a linear DNA plasmid fromXanthophyllomyces dendrorhous (Phaffia rhodozyma)

Microbiology / Medical Microbiology / Plasmids / Molecular cloning / Amino Acid Sequence / Base Sequence / BASIDIOMYCOTA / Base Sequence / BASIDIOMYCOTA

Identification of a nucleoside/nucleobase transporter from Plasmodium falciparum, a novel target for anti-malarial chemotherapy

Kinetics / Biological Sciences / Phylogeny / Xenopus / Animals / Biochemical / CHEMICAL SCIENCES / Molecular cloning / Protein Secondary Structure Prediction / Time Factors / Substrate Specificity / Plasmodium falciparum / Antimalarials / Protozoan Proteins / Amino Acid Sequence / Nucleosides / Cations / Hydrogen-Ion Concentration / Adenine / Biochemical / CHEMICAL SCIENCES / Molecular cloning / Protein Secondary Structure Prediction / Time Factors / Substrate Specificity / Plasmodium falciparum / Antimalarials / Protozoan Proteins / Amino Acid Sequence / Nucleosides / Cations / Hydrogen-Ion Concentration / Adenine

Cloning and functional expression of a complementary DNA encoding a mammalian nucleoside transport protein

Membrane Proteins / Biological Chemistry / Gene expression / Biological Sciences / Xenopus / Humans / Animals / Biological / CHEMICAL SCIENCES / Molecular cloning / Rats / Rat / Protein Conformation / Amino Acid Sequence / Base Sequence / Oocytes / Nucleosides / Humans / Animals / Biological / CHEMICAL SCIENCES / Molecular cloning / Rats / Rat / Protein Conformation / Amino Acid Sequence / Base Sequence / Oocytes / Nucleosides

Mammalian Expression of Cloned cDNA Sequences Encoding Human Purine Nucleoside Phosphorylase and Adenosine Deaminasea

Multidisciplinary / DNA / Cell line / Humans / Mice / Animals / Molecular cloning / Base Sequence / Adenosine Deaminase / Genetic Code / Purine Nucleoside Phosphorylase / Animals / Molecular cloning / Base Sequence / Adenosine Deaminase / Genetic Code / Purine Nucleoside Phosphorylase

Characterization of Mannuronan C-5-Epimerase Genes from the Brown Alga Laminaria digitata

Biological Sciences / Phylogeny / Plant Physiology / Sequence alignment / Plant / Molecular cloning / Protoplasts / Protein Conformation / Amino Acid Sequence / Recombinant Proteins / Molecular cloning / Protoplasts / Protein Conformation / Amino Acid Sequence / Recombinant Proteins

DNM1, a dynamin-related gene, participates in endosomal trafficking in yeast

Reproduction / Kinetics / Pheromones / Cell Biology / Biological Sciences / Saccharomyces cerevisiae / Mutation / G protein-coupled receptors / Endocytosis / Polymerase Chain Reaction / Genetic Map / Peptides / Molecular cloning / Fluorescent Antibody Technique / The cell / Amino Acid Profile / Amino Acid Sequence / Base Sequence / Ligands / Endosomes / Full Length Movies / Saccharomyces cerevisiae / Mutation / G protein-coupled receptors / Endocytosis / Polymerase Chain Reaction / Genetic Map / Peptides / Molecular cloning / Fluorescent Antibody Technique / The cell / Amino Acid Profile / Amino Acid Sequence / Base Sequence / Ligands / Endosomes / Full Length Movies

Isolation of cDNA libraries from individual human preimplantation embryos

Physiology / Morphogenesis / Humans / Polymerase Chain Reaction / Blastocyst / Clinical Sciences / Molecular cloning / Embryos / cDNA library / Clinical Sciences / Molecular cloning / Embryos / cDNA library

Effect of lambda packaging extract mcr restriction activity on DNA cloning

Biological Sciences / DNA / Environmental Sciences / Humans / Escherichia coli / Methylation / Animals / Nucleic Acids / Molecular cloning / Hybrid Solar Cells / Methylation / Animals / Nucleic Acids / Molecular cloning / Hybrid Solar Cells

Heterologous expression of the benzoate para-hydroxylase encoding gene (CYP53B1) from Rhodotorula minuta by Yarrowia lipolytica

Gene expression / Multidisciplinary / Genetic transformation / Enzyme / Applied Microbiology and Biotechnology / Molecular cloning / Benzoic Acid / Biotransformation / Genome sequence / Yarrowia / Stearic Acid / Heterologous Expression / Recombinant Proteins / Molecular cloning / Benzoic Acid / Biotransformation / Genome sequence / Yarrowia / Stearic Acid / Heterologous Expression / Recombinant Proteins

Novel 3-N-Aminoglycoside Acetyltransferase Gene, aac(3)-Ic, from a Pseudomonas aeruginosa Integron

Microbiology / Medical Microbiology / In Vitro / Humans / Escherichia coli / Resistance / Bacteria / Gene / Pseudomonas aeruginosa / Molecular cloning / Integrons / Gen / Genotype / Pseudomonas Aeruginosa / Biological activity / Amino Acid Sequence / Beta Lactamases / Resistance / Bacteria / Gene / Pseudomonas aeruginosa / Molecular cloning / Integrons / Gen / Genotype / Pseudomonas Aeruginosa / Biological activity / Amino Acid Sequence / Beta Lactamases

Expression of human relaxin genes: characterization of a novel alternatively-spliced human relaxin mRNA species

Gene expression / Biological Sciences / DNA / Molecular and cellular biology / Humans / Placenta / Female / Animals / Male / Polymerase Chain Reaction / Alternative splicing / Prostate / Gene Structure / Molecular cloning / Pan troglodytes / mRNA / Alternative Splicing / Amino Acid Sequence / Base Sequence / Ovary / Corpus Luteum / Relaxin / Placenta / Female / Animals / Male / Polymerase Chain Reaction / Alternative splicing / Prostate / Gene Structure / Molecular cloning / Pan troglodytes / mRNA / Alternative Splicing / Amino Acid Sequence / Base Sequence / Ovary / Corpus Luteum / Relaxin

Physarum polymalic acid hydrolase: Recombinant expression and enzyme activation

Biotechnology / Drug delivery / Polymers / Saccharomyces cerevisiae / Escherichia coli / Animals / Physarum Polycephalum / Protein Tyrosine Kinase / Molecular cloning / Enzyme activity / Protozoan Proteins / Recombinant Proteins / Plasma Membrane / Secretion / Glycosyl Hydrolases / Biochemistry and cell biology / Tyrosine Phosphorylation / Animals / Physarum Polycephalum / Protein Tyrosine Kinase / Molecular cloning / Enzyme activity / Protozoan Proteins / Recombinant Proteins / Plasma Membrane / Secretion / Glycosyl Hydrolases / Biochemistry and cell biology / Tyrosine Phosphorylation

Expression of 25 hydroxyvitamin D3-1α-hydroxylase in human endometrial tissue

Cancer / Treatment / Vitamin D / Cell line / Humans / Female / Alternative splicing / Molecular cloning / Alternative Splicing / Cell Proliferation / Micronutrient / Utero / Endometrium / Biochemistry and cell biology / Female / Alternative splicing / Molecular cloning / Alternative Splicing / Cell Proliferation / Micronutrient / Utero / Endometrium / Biochemistry and cell biology

DNA Polymerase lambda , a Novel DNA Repair Enzyme in Human Cells

Biological Chemistry / DNA repair / Biological Sciences / Humans / Biological / Enzyme / CHEMICAL SCIENCES / Molecular cloning / Protein Conformation / Amino Acid Sequence / Base Sequence / DNA Polymerase / Enzyme / CHEMICAL SCIENCES / Molecular cloning / Protein Conformation / Amino Acid Sequence / Base Sequence / DNA Polymerase

An in planta-expressed polyketide synthase produces (R)-mellein in the wheat pathogen Parastagonospora nodorum

Gene expression / Multidisciplinary / Saccharomyces cerevisiae / Phylogeny / Applied Environmental Microbiology / Medicago / Molecular cloning / Plant Development / Triticum / Ascomycota / Ochratoxins / Medicago / Molecular cloning / Plant Development / Triticum / Ascomycota / Ochratoxins

Mycoplasma synoviae surface protein MSPB as a recombinant antigen in an indirect ELISA

Microbiology / Multidisciplinary / Respiratory Disease / Escherichia coli / Animals / Polymerase Chain Reaction / Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis / Experimental Infection / Molecular cloning / POULTRY DISEASES / Chickens / Mycoplasma / Amino Acid Profile / Recombinant Proteins / Fusion Protein / Enzyme Linked Immunosorbent Assay / Immunoglobulin / Immunoblotting / Polymerase Chain Reaction / Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis / Experimental Infection / Molecular cloning / POULTRY DISEASES / Chickens / Mycoplasma / Amino Acid Profile / Recombinant Proteins / Fusion Protein / Enzyme Linked Immunosorbent Assay / Immunoglobulin / Immunoblotting
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