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Molecular cloning
Molecular cloning
Poly Specific trans -Acyltransferase Machinery Revealed via Engineered Acyl-CoA Synthetases
Natural Products / Biosynthesis of Natural Products / Macromolecular X-Ray Crystallography / Biological Sciences / High Performance Liquid Chromatography / CHEMICAL SCIENCES / Molecular cloning / Substrate Specificity / Polyketides / Genetic variation / Enzyme Engineering / Recombinant Proteins / CHEMICAL SCIENCES / Molecular cloning / Substrate Specificity / Polyketides / Genetic variation / Enzyme Engineering / Recombinant Proteins
Establishment of okadaic acid resistant cell clones using a cDNA expression library
Independent Component Analysis / Enzyme Inhibitors / Apoptosis / Biological Sciences / Caspases / Humans / Mice / Multidrug Resistance / Animals / Drug Resistance / Cell Death / Cytochrome C / Phenotype / Molecular cloning / Transfection / Protein Phosphorylation / Amino Acid Sequence / Base Sequence / Staurosporine / Cell Size / Okadaic acid / Molecular Sequence Data / fibroblasts / Humans / Mice / Multidrug Resistance / Animals / Drug Resistance / Cell Death / Cytochrome C / Phenotype / Molecular cloning / Transfection / Protein Phosphorylation / Amino Acid Sequence / Base Sequence / Staurosporine / Cell Size / Okadaic acid / Molecular Sequence Data / fibroblasts
Establishment of okadaic acid resistant cell clones using a cDNA expression library
Independent Component Analysis / Enzyme Inhibitors / Apoptosis / Biological Sciences / Caspases / Humans / Mice / Multidrug Resistance / Animals / Drug Resistance / Cell Death / Cytochrome C / Phenotype / Molecular cloning / Transfection / Protein Phosphorylation / Amino Acid Sequence / Base Sequence / Staurosporine / Cell Size / Okadaic acid / Molecular Sequence Data / fibroblasts / Humans / Mice / Multidrug Resistance / Animals / Drug Resistance / Cell Death / Cytochrome C / Phenotype / Molecular cloning / Transfection / Protein Phosphorylation / Amino Acid Sequence / Base Sequence / Staurosporine / Cell Size / Okadaic acid / Molecular Sequence Data / fibroblasts
Isolation of pig mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase gene promoter: characterization of a peroxisome proliferator-responsive element
Mitochondria / Transcription Factors / Biological Sciences / Animals / Biochemical / Lipid metabolism / CHEMICAL SCIENCES / Molecular cloning / Swine / Species Specificity / Amino Acid Sequence / Base Sequence / Protein Binding / Response Elements / Molecular Sequence Data / Lipid metabolism / CHEMICAL SCIENCES / Molecular cloning / Swine / Species Specificity / Amino Acid Sequence / Base Sequence / Protein Binding / Response Elements / Molecular Sequence Data
Immunotherapeutic potential of recombinant ESAT-6 protein in mouse model of experimental tuberculosis
Immunology / DDT / Humans / Mycobacterium tuberculosis / Escherichia coli / Mice / Female / Animals / Immunotherapy / Lung / Molecular cloning / Bacterial Load / Pulmonary Tuberculosis / Recombinant Proteins / Mice / Female / Animals / Immunotherapy / Lung / Molecular cloning / Bacterial Load / Pulmonary Tuberculosis / Recombinant Proteins
Pseudomonas exotoxin A-epidermal growth factor (EGF) mutant chimeric protein as an indicator for identifying amino acid residues important in EGF-receptor interaction
Biological Chemistry / Biological Sciences / Cell line / Humans / Bacterial Toxins / Escherichia coli / Biological / Plasmids / Pseudomonas aeruginosa / CHEMICAL SCIENCES / Molecular cloning / Epidermal Growth Factor / Pseudomonas Aeruginosa / Amino Acid Sequence / Base Sequence / Virulence Factors / Cell Survival / Site-directed Mutagenesis / Molecular Sequence Data / Escherichia coli / Biological / Plasmids / Pseudomonas aeruginosa / CHEMICAL SCIENCES / Molecular cloning / Epidermal Growth Factor / Pseudomonas Aeruginosa / Amino Acid Sequence / Base Sequence / Virulence Factors / Cell Survival / Site-directed Mutagenesis / Molecular Sequence Data
Characterization of Mannuronan C-5-Epimerase Genes from the Brown Alga Laminaria digitata
Biological Sciences / Phylogeny / Plant Physiology / Sequence alignment / Plant / Molecular cloning / Protoplasts / Protein Conformation / Amino Acid Sequence / Recombinant Proteins / Molecular Sequence Data / Molecular cloning / Protoplasts / Protein Conformation / Amino Acid Sequence / Recombinant Proteins / Molecular Sequence Data
A site-directed mutant of Cu,Zn-superoxide dismutase modeled after native extracellular superoxide dismutase
Genetics / Humans / Escherichia coli / Affinity chromatography / Superoxide Dismutase / Enzyme / Molecular cloning / Spectrum / Proteoglycans / Thermal Stability / Specific Activity / Arginine / Genetic variation / Amino Acid Profile / Glycine / Reperfusion injury / Amino Acid Sequence / Cell Surface Markers / Fusion Protein / Site-directed Mutagenesis / Molecular Sequence Data / Enzyme / Molecular cloning / Spectrum / Proteoglycans / Thermal Stability / Specific Activity / Arginine / Genetic variation / Amino Acid Profile / Glycine / Reperfusion injury / Amino Acid Sequence / Cell Surface Markers / Fusion Protein / Site-directed Mutagenesis / Molecular Sequence Data
Feline immunodeficiency virus subtypes A, B and C and intersubtype recombinants in Ontario, Canada
Geography / Cats / Biological Sciences / Phylogeny / Animals / Polymerase Chain Reaction / Ontario / Molecular cloning / Genetic Recombination / Base Sequence / Feline immunodeficiency virus / Molecular Sequence Data / Polymerase Chain Reaction / Ontario / Molecular cloning / Genetic Recombination / Base Sequence / Feline immunodeficiency virus / Molecular Sequence Data
Insights into Exo- and Endoglucanase Activities of Family 6 Glycoside Hydrolases from Podospora anserina
Multidisciplinary / Cellulase / Sequence alignment / Applied Environmental Microbiology / Polymerase Chain Reaction / Molecular cloning / Amino Acid Sequence / Molecular Sequence Data / Molecular cloning / Amino Acid Sequence / Molecular Sequence Data
Mycobacterium tuberculosis efpA encodes an efflux protein of the QacA transporter family
Immunology / Transcription Regulation / Tuberculosis / Humans / Mycobacterium tuberculosis / Drug Resistance / Polymerase Chain Reaction / Membrane transport proteins / Molecular cloning / Secondary Structure / Amino Acid Sequence / Base Sequence / Reverse Transcriptase / Mycobacterium bovis / Mycobacterium leprae / Mycobacterium avium / Molecular Sequence Data / Drug Resistance / Polymerase Chain Reaction / Membrane transport proteins / Molecular cloning / Secondary Structure / Amino Acid Sequence / Base Sequence / Reverse Transcriptase / Mycobacterium bovis / Mycobacterium leprae / Mycobacterium avium / Molecular Sequence Data
Plasmodium falciparum origin recognition complex subunit 5: functional characterization and role in DNA replication foci formation
DNA replication / Molecular Microbiology / Gene expression / Biological Sciences / Animals / Molecular cloning / Cell nucleus / Plasmodium falciparum / Protozoan Proteins / Amino Acid Sequence / Molecular cloning / Cell nucleus / Plasmodium falciparum / Protozoan Proteins / Amino Acid Sequence
An in planta-expressed polyketide synthase produces (R)-mellein in the wheat pathogen Parastagonospora nodorum
Gene expression / Multidisciplinary / Saccharomyces cerevisiae / Phylogeny / Applied Environmental Microbiology / Medicago / Molecular cloning / Plant Development / Triticum / Ascomycota / Ochratoxins / Molecular Sequence Data / Medicago / Molecular cloning / Plant Development / Triticum / Ascomycota / Ochratoxins / Molecular Sequence Data
Mn(II,III) oxidation and MnO2 mineralization by an expressed bacterial multicopper oxidase
Mass Spectrometry / X ray absorption spectroscopy / Multidisciplinary / Manganese / Escherichia coli / Bacillus / Oxidoreductases / Molecular cloning / Oxides / Manganese Compounds / Diphosphates / Oxidation-Reduction / Bacillus / Oxidoreductases / Molecular cloning / Oxides / Manganese Compounds / Diphosphates / Oxidation-Reduction
Transduction of an HLA-DP4???restricted NY-ESO-1???specific TCR into Primary Human CD4+ Lymphocytes
Immunology / Humans / Melanoma / Immunotherapy / Molecular cloning / Cell Proliferation / Epitopes / Cell Proliferation / Epitopes
Molecular characterization of two Brassica napus pollen-expressed genes encoding putative arabinogalactan proteins
Plant Biology / Gene expression / Biological Sciences / Plant Physiology / Pollen / Plant / Proteins / POLLEN GERMINATION / Glycoproteins / Molecular cloning / Germination / Molecular Characterization / Brassica / Brassica Napus / Androecium / Molecular Mass / Protein Conformation / Amino Acid Sequence / Base Sequence / Gene Family / PLANT PROTEINS / Pollen tube / Molecular Sequence Data / Plant / Proteins / POLLEN GERMINATION / Glycoproteins / Molecular cloning / Germination / Molecular Characterization / Brassica / Brassica Napus / Androecium / Molecular Mass / Protein Conformation / Amino Acid Sequence / Base Sequence / Gene Family / PLANT PROTEINS / Pollen tube / Molecular Sequence Data
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