Molecular cloning - Page 25

Production of l-asparaginase in Enterobacter aerogenes expressing Vitreoscilla hemoglobin for efficient oxygen uptake

Production / Gene expression / Multidisciplinary / Glucose / Bacteria / Oxygen Uptake / Ammonia / Enzyme / Applied Microbiology and Biotechnology / Molecular cloning / Enterobacter aerogenes / L-asparaginase / Enzyme activity / Recombinant Proteins / Hydrogen-Ion Concentration / Oxygen Consumption / Oxidation-Reduction / Hemoglobins / Oxygen Uptake / Ammonia / Enzyme / Applied Microbiology and Biotechnology / Molecular cloning / Enterobacter aerogenes / L-asparaginase / Enzyme activity / Recombinant Proteins / Hydrogen-Ion Concentration / Oxygen Consumption / Oxidation-Reduction / Hemoglobins

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Characterization of purified recombinant fibrinogen: partial phosphorylation of fibrinopeptide A

Biochemistry / Western blotting / DNA / Fibrinogen / Thrombin / Humans / Animals / Phosphorylation / Molecular cloning / Transfection / Base Sequence / Recombinant Proteins / Enzyme Linked Immunosorbent Assay / Biochemistry and cell biology / Molecular Sequence Data / Humans / Animals / Phosphorylation / Molecular cloning / Transfection / Base Sequence / Recombinant Proteins / Enzyme Linked Immunosorbent Assay / Biochemistry and cell biology / Molecular Sequence Data

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Biochemical Properties and Crystal Structure of a -Phenylalanine Aminotransferase from Variovorax paradoxus

Multidisciplinary / Macromolecular X-Ray Crystallography / Escherichia coli / Applied Environmental Microbiology / Temperature / Molecular cloning / Phenylalanine / Substrate Specificity / Protein Conformation / Molecular weight / Amino Acid Sequence / Recombinant Proteins / Transaminases / Molecular Sequence Data / Molecular cloning / Phenylalanine / Substrate Specificity / Protein Conformation / Molecular weight / Amino Acid Sequence / Recombinant Proteins / Transaminases / Molecular Sequence Data

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Tight transcriptional control mechanism ensures stable high-level expression from T7 promoter-based expression plasmids

Cytokines / DNA / Humans / Escherichia coli / Mice / Bio Technology / Animals / Plasmids / Molecular cloning / Tumor necrosis factor-alpha / Gene Expression Regulation / Bio Technology / Animals / Plasmids / Molecular cloning / Tumor necrosis factor-alpha / Gene Expression Regulation

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A 63 kDa VSP9B10A-like protein expressed in a C-8 Giardia duodenalis Mexican clone

Kinetics / Fluorescence Microscopy / Cell Adhesion / Adhesion / Western blotting / Mexico / DNA / Dogs / Cell line / Humans / Mice / Sheep / Animals / Polymerase Chain Reaction / Peptides / Giardiasis / Molecular cloning / Time Factors / Protein Expression / Genetic variation / MEXICO / Protozoan Proteins / Amino Acid Sequence / Base Sequence / Epitopes / Giardia / Antigenic Variation / Molecular Sequence Data / Child preschool / Mexico / DNA / Dogs / Cell line / Humans / Mice / Sheep / Animals / Polymerase Chain Reaction / Peptides / Giardiasis / Molecular cloning / Time Factors / Protein Expression / Genetic variation / MEXICO / Protozoan Proteins / Amino Acid Sequence / Base Sequence / Epitopes / Giardia / Antigenic Variation / Molecular Sequence Data / Child preschool

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Dengue-1 envelope protein domain III produced in Pichia pastoris: Potential use for serological diagnosis

Dengue Virus / Humans / Dengue / Expression / Molecular cloning / Protein expression and purification / Amino Acid Sequence / Base Sequence / Recombinant Proteins / Enzyme Linked Immunosorbent Assay / Biochemistry and cell biology / immunoglobulin G / Molecular Sequence Data / Protein expression and purification / Amino Acid Sequence / Base Sequence / Recombinant Proteins / Enzyme Linked Immunosorbent Assay / Biochemistry and cell biology / immunoglobulin G / Molecular Sequence Data

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Dictyostelium discoideum protein disulfide isomerase, an endoplasmic reticulum resident enzyme lacking a KDEL-type retrieval signal

Protein Folding / Sequence Analysis / Sequence alignment / Animals / Endoplasmic Reticulum / Dictyostelium / Enzyme / Steady state / Molecular cloning / Thioredoxin / Amino Acid Sequence / Biochemistry and cell biology / Molecular Sequence Data / Dictyostelium / Enzyme / Steady state / Molecular cloning / Thioredoxin / Amino Acid Sequence / Biochemistry and cell biology / Molecular Sequence Data

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Xenopus laevis Cu,Zn superoxide dismutase B cDNA sequence

Biological Sciences / DNA / Environmental Sciences / Animals / Nucleic Acids / Gene / Superoxide Dismutase / Molecular cloning / Gen / Xenopus laevis / Genetic variation / Amino Acid Sequence / Base Sequence / Molecular Sequence Data / Gene / Superoxide Dismutase / Molecular cloning / Gen / Xenopus laevis / Genetic variation / Amino Acid Sequence / Base Sequence / Molecular Sequence Data

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Xenopus laevis Cu,Zn superoxide dismutase B cDNA sequence

Biological Sciences / DNA / Environmental Sciences / Animals / Nucleic Acids / Gene / Superoxide Dismutase / Molecular cloning / Gen / Xenopus laevis / Genetic variation / Amino Acid Sequence / Base Sequence / Molecular Sequence Data / Gene / Superoxide Dismutase / Molecular cloning / Gen / Xenopus laevis / Genetic variation / Amino Acid Sequence / Base Sequence / Molecular Sequence Data

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Restoration of Nucleotide Excision Repair in a Helicase-Deficient XPD Mutant from Intragenic Suppression by a Trichothiodystrophy Mutation

Genetics / Cellular Biology / DNA repair / Transcription Factors / Western blotting / Biological Sciences / Cell line / Molecular and cellular biology / Mutation / Animals / Nucleotide Excision Repair / Proteins / Plasmids / Phenotype / Molecular cloning / Time Factors / Transfection / Structure activity Relationship / DNA binding proteins / Xeroderma Pigmentosum / Transcription Coupled Repair / Biological Sciences / Cell line / Molecular and cellular biology / Mutation / Animals / Nucleotide Excision Repair / Proteins / Plasmids / Phenotype / Molecular cloning / Time Factors / Transfection / Structure activity Relationship / DNA binding proteins / Xeroderma Pigmentosum / Transcription Coupled Repair

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Human xeroderma pigmentosum group D gene encodes a DMA helicase

Genetics / DNA repair / Transcription Factors / Multidisciplinary / Nature / Saccharomyces cerevisiae / Humans / Cell fusion / Skin Cancer / Escherichia coli / Ultraviolet / Nucleotide Excision Repair / Proteins / Molecular cloning / Human Disease / Recombinant Proteins / DNA binding proteins / Xeroderma Pigmentosum / Adenosine Triphosphate / Saccharomyces cerevisiae / Humans / Cell fusion / Skin Cancer / Escherichia coli / Ultraviolet / Nucleotide Excision Repair / Proteins / Molecular cloning / Human Disease / Recombinant Proteins / DNA binding proteins / Xeroderma Pigmentosum / Adenosine Triphosphate

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Retrotransposon-like sequences are expressed in Y chromosomal lampbrush loops of Drosophila hydei

Molecular Biology / Recombinant DNA Technology / Drosophila / Animals / Y chromosome / Molecular cloning / Genome Organization / Amino Acid Sequence / Base Sequence / Biochemistry and cell biology / Molecular Sequence Data / Molecular cloning / Genome Organization / Amino Acid Sequence / Base Sequence / Biochemistry and cell biology / Molecular Sequence Data

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Identification of cloned Y chromosomal DNA sequences from a lampbrush loop of Drosophila hydei

Multidisciplinary / Recombinant DNA Technology / DNA / Drosophila / Animals / Nucleic acid hybridization / Male / Sex chromosomes / Y chromosome / Molecular cloning / Molecular weight / DNA sequence / Nucleic acid hybridization / Male / Sex chromosomes / Y chromosome / Molecular cloning / Molecular weight / DNA sequence

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In vitro and in vivo comparison of two non-peptide tachykinin NK3 receptor antagonists: Improvements in efficacy achieved through enhanced brain penetration or altered pharmacological characteristics

Animal Behavior / Neurotransmission / Pharmacokinetics / Comparative Study / Prefrontal Cortex / Nucleus Accumbens / Dopamine / In Vitro / Brain / Humans / Cerebral Cortex / Animals / Male / Central Nervous System / Peptides / Habenula / Neurons / Efficiency / Haloperidol / European / Microdialysis / Molecular cloning / In Vivo / Rats / Neuronal Activity / Guinea Pig / Substance P / Quinolines / Extracellular Space / Pharmacokinetic / Medial Prefrontal Cortex / Clinical evaluation / Inositol Phosphates / Nucleus Accumbens / Dopamine / In Vitro / Brain / Humans / Cerebral Cortex / Animals / Male / Central Nervous System / Peptides / Habenula / Neurons / Efficiency / Haloperidol / European / Microdialysis / Molecular cloning / In Vivo / Rats / Neuronal Activity / Guinea Pig / Substance P / Quinolines / Extracellular Space / Pharmacokinetic / Medial Prefrontal Cortex / Clinical evaluation / Inositol Phosphates

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Screening and its potential application of lipolytic activity from a marine environment: characterization of a novel esterase from Yarrowia lipolytica CL180

Applied microbiology / Multidisciplinary / Sequence Analysis / Screening / Sequence alignment / Escherichia coli / Animals / Lipolysis / Temperature / Application / Applied Microbiology and Biotechnology / Molecular cloning / Hydrothermal Vent / Marine Environment / Substrate Specificity / Fatty Acid / Genomic Library / Tidal Flat / Amino Acid Profile / Molecular Mass / Water Microbiology / Esterases / Molecular weight / Amino Acid Sequence / Open Reading Frame / Ofloxacin / Yarrowia / Recombinant Protein / Recombinant Proteins / Hydrogen-Ion Concentration / Levofloxacin / Thallophyta / Molecular Sequence Data / Yarrowia Lipolytica / Escherichia coli / Animals / Lipolysis / Temperature / Application / Applied Microbiology and Biotechnology / Molecular cloning / Hydrothermal Vent / Marine Environment / Substrate Specificity / Fatty Acid / Genomic Library / Tidal Flat / Amino Acid Profile / Molecular Mass / Water Microbiology / Esterases / Molecular weight / Amino Acid Sequence / Open Reading Frame / Ofloxacin / Yarrowia / Recombinant Protein / Recombinant Proteins / Hydrogen-Ion Concentration / Levofloxacin / Thallophyta / Molecular Sequence Data / Yarrowia Lipolytica

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RNA-Catalyzed Amino Acid Activation †

Biochemistry / Catalysis / High Pressure Liquid Chromatography / Molecular cloning / Amino Acids / Guanosine Triphosphate / Amino Acid Profile / Carboxylic Acids / Base Sequence / Diphosphates / Nucleic Acid Conformation / Biochemistry and cell biology / Molecular Sequence Data / Guanosine Triphosphate / Amino Acid Profile / Carboxylic Acids / Base Sequence / Diphosphates / Nucleic Acid Conformation / Biochemistry and cell biology / Molecular Sequence Data

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