Inhibition of CXCL12/CXCR4 autocrine/paracrine loop reduces viability of human glioblastoma stem-like cells affecting self-renewal activity

SUPPLEMENTARY MATERIAL TO INHIBITION OF CXCL12/CXCR4 AUTOCRINE/PARACRINE LOOP REDUCES VIABILITY OF HUMAN GLIOBLASTOMA STEM-LIKE CELLS AFFECTING SELF-RENEWAL ACTIVITY by Monica Gatti, Alessandra Pattarozzi, Adriana Bajetto, Roberto Würth, Antonio Daga, Pietro Fiaschi, Gianluigi Zona, Tullio Florio and Federica Barbieri

Supplementary Table 1 Characteristics of patients and tumors from which GBM CSCs were isolated. Code

Age Sex WHO grade

GBM1 67 GBM2 48 GBM3 40

M M F

GBM4 71

F

GBM5 70

M

IV (primary) IV (primary) IV (secondary, progress from oligodendroglioma) IV (primary, multicentric) IV (primary)

IHC

MIBIndex (%) GFAP+ 40 GFAP+ 60 GFAP+ 30

Cerebral emisphere/lobe*

Localization Meningeal infiltration

Right/T-P Left/T Right/F-T

Cortical Subcortical Corticalsubcortical

no no yes

GFAP+ 30

Left/T-P-O

no

GFAP+ 40

Left/F

Corticalsubcortical Subcortical

*T: temporal; P: parietal; F: frontal; O: occipital

no

Supplementary Figure 1

Supplementary Figure 1 Legend Lack of EGFR transactivation by CXCL12 treatment in human GBM CSC cultures. EGFR transactivation was evaluated by Western blot using anti-phospho-EGFR (Tyr-1173) antibody. EGF treatment was used as positive control, and total EGFR and -tubulin were used as controls of total protein loaded in the gel.