Site-directed Mutagenesis - Page 4

Mechanism of phosphoryl transfer by nucleoside diphosphate kinase

Catalysis / Kinetics / Macromolecular X-Ray Crystallography / Phosphorus / Animals / Dictyostelium / Active site / Lysine / Nucleoside Diphosphate Kinase / Protein Conformation / Structure activity Relationship / Hydrogen-Ion Concentration / Asparagine / Site-directed Mutagenesis / Tyrosine / Biochemistry and cell biology / Adenosine Triphosphate / Dictyostelium / Active site / Lysine / Nucleoside Diphosphate Kinase / Protein Conformation / Structure activity Relationship / Hydrogen-Ion Concentration / Asparagine / Site-directed Mutagenesis / Tyrosine / Biochemistry and cell biology / Adenosine Triphosphate

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An Internalization Signal in ClC-5, an Endosomal Cl- Channel Mutated in Dent\'s Disease

Kidney diseases / Biological Chemistry / Biological Sciences / Mice / Endocytosis / Animals / CHEMICAL SCIENCES / Xenopus laevis / Amino Acid Sequence / Site-directed Mutagenesis / Endosomes / Molecular Sequence Data / Animals / CHEMICAL SCIENCES / Xenopus laevis / Amino Acid Sequence / Site-directed Mutagenesis / Endosomes / Molecular Sequence Data

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Deficiency of dolichyl-P-Man:Man7GlcNAc2-PP-dolichyl mannosyltransferase causes congenital disorder of glycosylation type Ig

Biological Sciences / Humans / Female / Endoplasmic Reticulum / Transferrin / Biochemical / Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis / Phenotype / Glycosylation / High Pressure Liquid Chromatography / CHEMICAL SCIENCES / Site-directed Mutagenesis / Oligosaccharides / DNA mutational analysis / Biochemical / Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis / Phenotype / Glycosylation / High Pressure Liquid Chromatography / CHEMICAL SCIENCES / Site-directed Mutagenesis / Oligosaccharides / DNA mutational analysis

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Two different genes from Schwanniomyces occidentalis determine ribosomal resistance to cycloheximide

Humans / Animals / Microbial genetic and drug resistance / Polymerase Chain Reaction / Ribosomal proteins / Plasmids / Amino Acid Sequence / Base Sequence / Ribosomes / Site-directed Mutagenesis / Biochemistry and cell biology / Cycloheximide / Molecular Sequence Data / Plasmids / Amino Acid Sequence / Base Sequence / Ribosomes / Site-directed Mutagenesis / Biochemistry and cell biology / Cycloheximide / Molecular Sequence Data

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Clostridium perfringens epsilon toxin mutant Y30A-Y196A as a recombinant vaccine candidate against enterotoxemia

Biological Sciences / Dogs / Bacterial Toxins / Mice / Female / Animals / Vaccine / Rabbits / Recombinant Proteins / Site-directed Mutagenesis / Animals / Vaccine / Rabbits / Recombinant Proteins / Site-directed Mutagenesis

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Ion channels as devices

Molecular Biology / Molecular Biophysics / Genetic Engineering / Proteins / Microorganisms / Model System / Membrane Protein / Site-directed Mutagenesis / Chemical Modification / Model System / Membrane Protein / Site-directed Mutagenesis / Chemical Modification

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Exome sequencing identifies mutation in CNOT3 and ribosomal genes RPL5 and RPL10 in T-cell acute lymphoblastic leukemia

Drosophila melanogaster / Transcription Factors / Biological Sciences / Saccharomyces cerevisiae / RNA interference / Humans / Sequence alignment / Mutation / Mice / Animals / Ribosomal proteins / Base Sequence / Site-directed Mutagenesis / Exome / Polyribosomes / Molecular Sequence Data / Humans / Sequence alignment / Mutation / Mice / Animals / Ribosomal proteins / Base Sequence / Site-directed Mutagenesis / Exome / Polyribosomes / Molecular Sequence Data

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Arabidopsis KEA2, a homolog of bacterial KefC, encodes a K+/H+ antiporter with a chloroplast transit peptide

Chemical Engineering / Fluorescence Microscopy / Biological Sciences / Saccharomyces cerevisiae / Escherichia coli / Physical sciences / Peptides / Nickel / Affinity chromatography / Arabidopsis / Molecular cloning / Potassium Channels / Amino Acid Sequence / Cations / Site-directed Mutagenesis / Biochemistry and cell biology / Chloroplasts / Molecular Sequence Data / Physical sciences / Peptides / Nickel / Affinity chromatography / Arabidopsis / Molecular cloning / Potassium Channels / Amino Acid Sequence / Cations / Site-directed Mutagenesis / Biochemistry and cell biology / Chloroplasts / Molecular Sequence Data

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Poliovirus Polymerase Residue 5 Plays a Critical Role in Elongation Complex Stability

Virology / Biological Sciences / Humans / Virus / Amino Acid Substitution Rates / Site-directed Mutagenesis / Poliovirus / Site-directed Mutagenesis / Poliovirus

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Human Mitochondrial Manganese Superoxide Dismutase Polymorphic Variant Ile58Thr Reduces Activity by Destabilizing the Tetrameric Interface † , ‡

Biochemistry / Polymorphism / Electrochemistry / Oxidative Stress / Amyotrophic Lateral Sclerosis / Free Radical / Crystal structure / Humans / Temperature / Superoxide Dismutase / Phenotype / Enzyme / Body Temperature / Temperature Dependence / Active site / Thermal Stability / Oxidative Damage / Genetic variation / Enzyme activity / Protein Conformation / Elevated Temperature / Site-directed Mutagenesis / Nervous System Diseases / Biochemistry and cell biology / Molecular Structure / Melting Temperature / Molecular Sequence Data / Free Radical / Crystal structure / Humans / Temperature / Superoxide Dismutase / Phenotype / Enzyme / Body Temperature / Temperature Dependence / Active site / Thermal Stability / Oxidative Damage / Genetic variation / Enzyme activity / Protein Conformation / Elevated Temperature / Site-directed Mutagenesis / Nervous System Diseases / Biochemistry and cell biology / Molecular Structure / Melting Temperature / Molecular Sequence Data

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In vitro expression of natural mutants of human lecithin:cholesterol acyltransferase

Western blotting / Humans / Eye Disease / Lipid metabolism / Corneal Opacity / Specific Activity / Western blot / High Density Lipoprotein / Lipid / Base Sequence / Site-directed Mutagenesis / Low Density Lipoprotein (LDL) / Biochemistry and cell biology / Molecular Sequence Data / Specific Activity / Western blot / High Density Lipoprotein / Lipid / Base Sequence / Site-directed Mutagenesis / Low Density Lipoprotein (LDL) / Biochemistry and cell biology / Molecular Sequence Data

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Structural ( )8 TIM Barrel Model of 3-Hydroxy-3-methylglutaryl-Coenzyme A Lyase

Protein Folding / Biological Chemistry / Gene expression / Biological Sciences / Humans / Sequence alignment / Escherichia coli / Female / Animals / Male / Biological / Infant / Newborn Infant / CHEMICAL SCIENCES / Protein Secondary Structure Prediction / Amino Acid Sequence / Recombinant Proteins / Site-directed Mutagenesis / Molecular Structure / Molecular Sequence Data / Sequence alignment / Escherichia coli / Female / Animals / Male / Biological / Infant / Newborn Infant / CHEMICAL SCIENCES / Protein Secondary Structure Prediction / Amino Acid Sequence / Recombinant Proteins / Site-directed Mutagenesis / Molecular Structure / Molecular Sequence Data

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Genetic basis of mitochondrial HMG-CoA synthase deficiency

Genetics / Human Genetics / Complementary and Alternative Medicine / Mitochondria / Gene expression / Humans / Female / Animals / Male / Infant / Base Sequence / Site-directed Mutagenesis / DNA mutational analysis / Humans / Female / Animals / Male / Infant / Base Sequence / Site-directed Mutagenesis / DNA mutational analysis

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Pseudomonas exotoxin A-epidermal growth factor (EGF) mutant chimeric protein as an indicator for identifying amino acid residues important in EGF-receptor interaction

Biological Chemistry / Biological Sciences / Cell line / Humans / Bacterial Toxins / Escherichia coli / Biological / Plasmids / Pseudomonas aeruginosa / CHEMICAL SCIENCES / Molecular cloning / Epidermal Growth Factor / Pseudomonas Aeruginosa / Amino Acid Sequence / Base Sequence / Virulence Factors / Cell Survival / Site-directed Mutagenesis / Molecular Sequence Data / Escherichia coli / Biological / Plasmids / Pseudomonas aeruginosa / CHEMICAL SCIENCES / Molecular cloning / Epidermal Growth Factor / Pseudomonas Aeruginosa / Amino Acid Sequence / Base Sequence / Virulence Factors / Cell Survival / Site-directed Mutagenesis / Molecular Sequence Data

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A site-directed mutant of Cu,Zn-superoxide dismutase modeled after native extracellular superoxide dismutase

Genetics / Humans / Escherichia coli / Affinity chromatography / Superoxide Dismutase / Enzyme / Molecular cloning / Spectrum / Proteoglycans / Thermal Stability / Specific Activity / Arginine / Genetic variation / Amino Acid Profile / Glycine / Reperfusion injury / Amino Acid Sequence / Cell Surface Markers / Fusion Protein / Site-directed Mutagenesis / Molecular Sequence Data / Enzyme / Molecular cloning / Spectrum / Proteoglycans / Thermal Stability / Specific Activity / Arginine / Genetic variation / Amino Acid Profile / Glycine / Reperfusion injury / Amino Acid Sequence / Cell Surface Markers / Fusion Protein / Site-directed Mutagenesis / Molecular Sequence Data

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HLA-DPp residue 69 plays a crucial role in allorecognition

Immunology / Polymorphism / Clinical Sciences / Tissue / Allorecognition / Epitopes / Site-directed Mutagenesis / Epitopes / Site-directed Mutagenesis

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