Site-directed Mutagenesis - Page 2

Transforming a fructan:fructan 6G-fructosyltransferase from perennial ryegrass into a sucrose:sucrose 1-fructosyltransferase

Kinetics / Biological Sciences / Plant Physiology / Sequence alignment / Plant / Molecular cloning / Substrate Specificity / Amino Acid Sequence / PLANT PROTEINS / Site-directed Mutagenesis / Molecular cloning / Substrate Specificity / Amino Acid Sequence / PLANT PROTEINS / Site-directed Mutagenesis

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Human immunodeficiency virus type 1 Vpr: oligomerization is an essential feature for its incorporation into virus particles

Microbiology / Medical Microbiology / Virology / Molecular modeling / HIV / Higher Order Thinking / Cell line / Humans / Sequence alignment / Human immunodeficiency virus / Amino Acid Profile / Molecular Conformation / Amino Acid Sequence / Virus infection / Intermolecular Interaction / Site-directed Mutagenesis / Higher Order Thinking / Cell line / Humans / Sequence alignment / Human immunodeficiency virus / Amino Acid Profile / Molecular Conformation / Amino Acid Sequence / Virus infection / Intermolecular Interaction / Site-directed Mutagenesis

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Hypersensitivity to seizures in beta-amyloid precursor protein deficient mice

Death / Apoptosis / Biological Sciences / Brain / Cerebral Cortex / Mice / Animals / Cell Death / Neurons / Seizures / Cell Survival / Site-directed Mutagenesis / Glutamate Receptor / Amyloid Beta Precursor Protein / Mice / Animals / Cell Death / Neurons / Seizures / Cell Survival / Site-directed Mutagenesis / Glutamate Receptor / Amyloid Beta Precursor Protein

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Exome sequencing identifies mutation in CNOT3 and ribosomal genes RPL5 and RPL10 in T-cell acute lymphoblastic leukemia

Drosophila melanogaster / Transcription Factors / Biological Sciences / Saccharomyces cerevisiae / RNA interference / Humans / Sequence alignment / Mutation / Mice / Animals / Ribosomal proteins / Base Sequence / Site-directed Mutagenesis / Exome / Polyribosomes / Humans / Sequence alignment / Mutation / Mice / Animals / Ribosomal proteins / Base Sequence / Site-directed Mutagenesis / Exome / Polyribosomes

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Structural and functional characterisation of an influenza virus RNA polymerase-genomic RNA complex

Microbiology / Zoology / Immunology / Molecular Biology / Structural Biology / Biomaterials / Tissue Engineering / Medical Microbiology / Virology / Wastewater Treatment / Hydrogels / Transcription Factors / Multidisciplinary / Extracellular Matrix / Animal Production / Molecular Mechanics / Enzyme Kinetics / Immunogenetics / Catalytic Mechanism / Biological Sciences / Protein Structure and Function / Crystal structure / Molecular Immunology / Heparin / Sequence alignment / Basement Membrane / Biological Wastewater Treatment / Mice / Animals / Blood / Polymerase Chain Reaction / Physical sciences / Special functions / Detoxification / Fisheries Sciences / Fibroblast Growth Factor / Glycosaminoglycan / Transcription Factor / Enzyme / Clinical Sciences / Active site / Polyvinyl alcohol / Poly Vinyl Alcohol / Amino Acid Profile / Veterinary Sciences / Biological activity / Amino Acid Sequence / Base Sequence / Materials Testing / Growth Factor / Structure activity Relationship / Protein Binding / D structure / Biocompatible Materials / Cell Surface Markers / Cardiovascular Magnetic Resonance / Site-directed Mutagenesis / Binding Site / Biochemistry and cell biology / Biomechanical Phenomena / Biomaterials / Tissue Engineering / Medical Microbiology / Virology / Wastewater Treatment / Hydrogels / Transcription Factors / Multidisciplinary / Extracellular Matrix / Animal Production / Molecular Mechanics / Enzyme Kinetics / Immunogenetics / Catalytic Mechanism / Biological Sciences / Protein Structure and Function / Crystal structure / Molecular Immunology / Heparin / Sequence alignment / Basement Membrane / Biological Wastewater Treatment / Mice / Animals / Blood / Polymerase Chain Reaction / Physical sciences / Special functions / Detoxification / Fisheries Sciences / Fibroblast Growth Factor / Glycosaminoglycan / Transcription Factor / Enzyme / Clinical Sciences / Active site / Polyvinyl alcohol / Poly Vinyl Alcohol / Amino Acid Profile / Veterinary Sciences / Biological activity / Amino Acid Sequence / Base Sequence / Materials Testing / Growth Factor / Structure activity Relationship / Protein Binding / D structure / Biocompatible Materials / Cell Surface Markers / Cardiovascular Magnetic Resonance / Site-directed Mutagenesis / Binding Site / Biochemistry and cell biology / Biomechanical Phenomena

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Designer laccases: a vogue for high-potential fungal enzymes?

Engineering / Technology / Gene expression / Protein Engineering / Genetic Engineering / Biological Sciences / Industrial microbiology / TRENDS / Laccase / Enzyme / Industrial Production / Recombinant Proteins / Ascomycota / Site-directed Mutagenesis / BASIDIOMYCOTA / Biological Sciences / Industrial microbiology / TRENDS / Laccase / Enzyme / Industrial Production / Recombinant Proteins / Ascomycota / Site-directed Mutagenesis / BASIDIOMYCOTA

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Mutagenesis of important amino acid reveals unconventional homologous internalization of β1-adrenergic receptor

Life Sciences / Western blotting / Cell line / Humans / Mutation / cyclic AMP / D-Aspartic Acid / Lysine / Radioligand Assay / Amino Acid Profile / Transfection / Propranolol / Site-directed Mutagenesis / Ligands / Biochemistry and cell biology / Isoproterenol / cyclic AMP / D-Aspartic Acid / Lysine / Radioligand Assay / Amino Acid Profile / Transfection / Propranolol / Site-directed Mutagenesis / Ligands / Biochemistry and cell biology / Isoproterenol

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Identification of key amino acid residues in Neisseria polysaccharea amylosucrase

Catalysis / Glycoside hydrolase / Protein Structure and Function / Sequence alignment / Glutathione / Neisseria / D-Aspartic Acid / Enzyme / Amino Acids / Amino Acid Profile / Amino Acid Sequence / Base Sequence / Structure activity Relationship / Polyacrylamide Gel Electrophoresis / Enzymatic Activity / Site-directed Mutagenesis / Glutamic Acid / Histidine / Biochemistry and cell biology / Neisseria / D-Aspartic Acid / Enzyme / Amino Acids / Amino Acid Profile / Amino Acid Sequence / Base Sequence / Structure activity Relationship / Polyacrylamide Gel Electrophoresis / Enzymatic Activity / Site-directed Mutagenesis / Glutamic Acid / Histidine / Biochemistry and cell biology

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Evaluation by site-directed mutagenesis of active site amino acid residues of Anaerobiospirillum succiniciproducens phosphoenolpyruvate carboxykinase

Carbon Dioxide / Kinetics / Protein Chemistry / Circular Dichroism / Escherichia coli / Enzyme / Protein Interaction / Active site / Amino Acid Profile / Metal ion / Recombinant Proteins / Site-directed Mutagenesis / Biochemistry and cell biology / Adenosine Triphosphate / Adenosine Diphosphate / Proteobacteria / Enzyme / Protein Interaction / Active site / Amino Acid Profile / Metal ion / Recombinant Proteins / Site-directed Mutagenesis / Biochemistry and cell biology / Adenosine Triphosphate / Adenosine Diphosphate / Proteobacteria

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Evaluation by Site-Directed Mutagenesis of Active Site Amino Acid Residues of Anaerobiospirillum succiniciproducens Phosphoenolpyruvate Carboxykinase

Carbon Dioxide / Kinetics / Protein Chemistry / Circular Dichroism / Escherichia coli / Enzyme / Protein Interaction / Active site / Amino Acid Profile / Metal ion / Recombinant Proteins / Site-directed Mutagenesis / Biochemistry and cell biology / Adenosine Triphosphate / Adenosine Diphosphate / Proteobacteria / Enzyme / Protein Interaction / Active site / Amino Acid Profile / Metal ion / Recombinant Proteins / Site-directed Mutagenesis / Biochemistry and cell biology / Adenosine Triphosphate / Adenosine Diphosphate / Proteobacteria

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Relative Replicative Fitness of Human Immunodeficiency Virus Type 1 Mutants Resistant to Enfuvirtide (T-20)

Virology / HIV / Biological Sciences / Cell line / Humans / Mutation / Human immunodeficiency virus / Heptad Repeat / Genetic Recombination / Site-directed Mutagenesis / In Vitro Selection / Full Length Movies / Mutation / Human immunodeficiency virus / Heptad Repeat / Genetic Recombination / Site-directed Mutagenesis / In Vitro Selection / Full Length Movies

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Farnesyl Protein Transferase: Identification of K164α and Y300β as Catalytic Residues by Mutagenesis and Kinetic Studies †

Biochemistry / Catalysis / Kinetics / Animals / Lysine / Rats / Hydrogen-Ion Concentration / Site-directed Mutagenesis / Tyrosine / Biochemistry and cell biology / Rats / Hydrogen-Ion Concentration / Site-directed Mutagenesis / Tyrosine / Biochemistry and cell biology

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Farnesyl Protein Transferase: Identification of K164α and Y300β as Catalytic Residues by Mutagenesis and Kinetic Studies †

Biochemistry / Catalysis / Kinetics / Animals / Lysine / Rats / Hydrogen-Ion Concentration / Site-directed Mutagenesis / Tyrosine / Biochemistry and cell biology / Rats / Hydrogen-Ion Concentration / Site-directed Mutagenesis / Tyrosine / Biochemistry and cell biology

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ModA and ModB, Two ADP-Ribosyltransferases Encoded by Bacteriophage T4: Catalytic Properties and Mutation Analysis

Microbiology / Bacteriology / RNA polymerase / Biological Sciences / Mutation / Virus / Enzyme / Inclusion Bodies / Secondary Structure / Amino Acid Profile / Amino Acid Sequence / Site-directed Mutagenesis / In Vitro Transcription / Virus / Enzyme / Inclusion Bodies / Secondary Structure / Amino Acid Profile / Amino Acid Sequence / Site-directed Mutagenesis / In Vitro Transcription

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Salmonella enterica Serovar Typhimurium blaPER-1-Carrying Plasmid pSTI1 Encodes an Extended-Spectrum Aminoglycoside 6\'-N-Acetyltransferase of Type Ib

Microbiology / Medical Microbiology / Bacterial resistance / Salmonella Typhimurium / Aminoglycosides / Plasmids / Enzyme / Spectrum / Anti-Bacterial Agents / Substrate Specificity / Amino Acid Profile / SIGNAL PEPTIDE / Amino Acid Sequence / Base Sequence / Open Reading Frame / Enzymatic Activity / Site-directed Mutagenesis / Nucleotides / Plasmids / Enzyme / Spectrum / Anti-Bacterial Agents / Substrate Specificity / Amino Acid Profile / SIGNAL PEPTIDE / Amino Acid Sequence / Base Sequence / Open Reading Frame / Enzymatic Activity / Site-directed Mutagenesis / Nucleotides

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Human DNMT2 methylates tRNAAsp molecules using a DNA methyltransferase-like catalytic mechanism

RNA / Drosophila melanogaster / Catalytic Mechanism / Humans / Sequence alignment / Animals / Dictyostelium / RNA isolation / Enzyme / Amino Acid Sequence / Knock out / Site-directed Mutagenesis / Catalytic Activity / Biochemistry and cell biology / Animals / Dictyostelium / RNA isolation / Enzyme / Amino Acid Sequence / Knock out / Site-directed Mutagenesis / Catalytic Activity / Biochemistry and cell biology

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