Site-directed Mutagenesis - Page 3

Identification of Escherichia coli mismatch-specific uracil DNA glycosylase as a robust xanthine DNA glycosylase

Kinetics / Biological Chemistry / DNA repair / Biological Sciences / Humans / Escherichia coli / CHEMICAL SCIENCES / Hydrogen Bonding / Amino Acid Sequence / Site-directed Mutagenesis / Escherichia coli / CHEMICAL SCIENCES / Hydrogen Bonding / Amino Acid Sequence / Site-directed Mutagenesis

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Functional Characterization of Gne (UDP-N-Acetylglucosamine- 4-Epimerase), Wzz (Chain Length Determinant), and Wzy (O-Antigen Polymerase) of Yersinia enterocolitica Serotype O:8

Genetics / Bacteriology / Biological Sciences / Lipopolysaccharide / Crystal structure / Escherichia coli / Enzyme / Gram-negative bacteria / Lipopolysaccharides / Active site / Site-directed Mutagenesis / Yersinia Enterocolitica / Escherichia coli / Enzyme / Gram-negative bacteria / Lipopolysaccharides / Active site / Site-directed Mutagenesis / Yersinia Enterocolitica

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Functional Characterization of Candida albicans ABC Transporter Cdr1p

Fluorescent Dyes and Reagents / Confocal Microscopy / Biological Sciences / Saccharomyces cerevisiae / Drug Resistance / P-glycoprotein / Candida albicans / Monoclonal Antibodies / Green Fluorescent Protein / Substrate Specificity / Microbial Sensitivity Tests / Genetic variation / Amino Acid Sequence / Amino Acid Substitution Rates / Fusion Protein / Site-directed Mutagenesis / Plasma Membrane / Binding Site / Eukaryotic Cell / Cycloheximide / P-glycoprotein / Candida albicans / Monoclonal Antibodies / Green Fluorescent Protein / Substrate Specificity / Microbial Sensitivity Tests / Genetic variation / Amino Acid Sequence / Amino Acid Substitution Rates / Fusion Protein / Site-directed Mutagenesis / Plasma Membrane / Binding Site / Eukaryotic Cell / Cycloheximide

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Alanine scanning of transmembrane helix 11 of Cdr1p ABC antifungal efflux pump of Candida albicans: identification of amino acid residues critical for drug efflux

Microbiology / Medical Microbiology / Multidrug Resistance / Candida albicans / Antimicrobial chemotherapy / Ketoconazole / Membrane transport proteins / Protein Secondary Structure Prediction / Substrate Specificity / Antifungal Agents / Amino Acid Profile / Efflux Pumps / Amino Acid Sequence / Base Sequence / Amino Acid Substitution Rates / Site-directed Mutagenesis / Ketoconazole / Membrane transport proteins / Protein Secondary Structure Prediction / Substrate Specificity / Antifungal Agents / Amino Acid Profile / Efflux Pumps / Amino Acid Sequence / Base Sequence / Amino Acid Substitution Rates / Site-directed Mutagenesis

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In Vitro Activities of the Multifunctional RNA Silencing Polymerase QDE-1 of Neurospora crassa

Biological Chemistry / RNA silencing / Neurospora crassa / Biological Sciences / CHEMICAL SCIENCES / Substrate Specificity / Structure activity Relationship / Site-directed Mutagenesis / Substrate Specificity / Structure activity Relationship / Site-directed Mutagenesis

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Adaptor Protein Lad Relays PDGF Signal to Grb2 in Lung Cells: A Tissue-Specific PDGF Signal Transduction

Signal Transduction / Cell line / Humans / Mice / Animals / Phosphorylation / T cells / Biochemical / Proteins / Lung / Bronchial Epithelial Cells / GRB / Site-directed Mutagenesis / Biochemistry and cell biology / Tissue Specificity / Tyrosine Phosphorylation / Phosphorylation / T cells / Biochemical / Proteins / Lung / Bronchial Epithelial Cells / GRB / Site-directed Mutagenesis / Biochemistry and cell biology / Tissue Specificity / Tyrosine Phosphorylation

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A proximal tryptophan in NO synthase controls activity by a novel mechanism

Inorganic Chemistry / Kinetics / Cytochrome C / Spectrophotometry / Tryptophan / Heme / Enzyme / Steady state / Hydrogen Bond / Hydrogen Bonding / Nitric Oxide Synthase / Cysteine / Molecular Conformation / Protein Conformation / Inorganic Biochemistry / Amino Acid Substitution Rates / Recombinant Proteins / Site-directed Mutagenesis / Binding affinity / Heme / Enzyme / Steady state / Hydrogen Bond / Hydrogen Bonding / Nitric Oxide Synthase / Cysteine / Molecular Conformation / Protein Conformation / Inorganic Biochemistry / Amino Acid Substitution Rates / Recombinant Proteins / Site-directed Mutagenesis / Binding affinity

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Mutagenesis of important amino acid reveals unconventional homologous internalization of β1-adrenergic receptor

Life Sciences / Western blotting / Internalization / Cell line / Humans / Mutation / cyclic AMP / D-Aspartic Acid / Lysine / Radioligand Assay / Amino Acid Profile / Transfection / Propranolol / Site-directed Mutagenesis / Ligands / Biochemistry and cell biology / Isoproterenol / Mutation / cyclic AMP / D-Aspartic Acid / Lysine / Radioligand Assay / Amino Acid Profile / Transfection / Propranolol / Site-directed Mutagenesis / Ligands / Biochemistry and cell biology / Isoproterenol

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A two-step recognition of signal sequences determines the translocation efficiency of proteins

Biological Sciences / DNA / Humans / Prolactin / Mice / Female / Animals / Chickens / Amino Acid Sequence / Base Sequence / Site-directed Mutagenesis / Plasminogen Activator Inhibitor / Female / Animals / Chickens / Amino Acid Sequence / Base Sequence / Site-directed Mutagenesis / Plasminogen Activator Inhibitor

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Identification of Trp300 as an Important Residue for Escherichia Coli Leader Peptidase Activity

Kinetics / Membrane Proteins / Mutation / Escherichia coli / Tryptophan / Molecular cloning / Active site / Base Sequence / Site-directed Mutagenesis / Hot Temperature / Biochemistry and cell biology / Molecular cloning / Active site / Base Sequence / Site-directed Mutagenesis / Hot Temperature / Biochemistry and cell biology

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Complementary regulation of anaesthetic activation of human (alpha6beta3gamma2L) and Drosophila (RDL) GABA receptors by a single amino acid residue

Electrophysiology / Patch-clamp and imaging techniques / Biological Sciences / Drosophila / Humans / GABA receptors / Animals / The / Propofol / Amino Acids / Xenopus laevis / Biotransformation / Oocytes / Site-directed Mutagenesis / Gamma-Aminobutyric Acid / Pentobarbital / GABA receptors / Animals / The / Propofol / Amino Acids / Xenopus laevis / Biotransformation / Oocytes / Site-directed Mutagenesis / Gamma-Aminobutyric Acid / Pentobarbital

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HLA-DPp residue 69 plays a crucial role in allorecognition

Immunology / Polymorphism / Clinical Sciences / Tissue / Allorecognition / Epitopes / Site-directed Mutagenesis / Epitopes / Site-directed Mutagenesis

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Ultrastable cellulosome-adhesion complex tightens under load

Biophysics / Molecular Dynamics Simulation / Protein Folding / Catalysis / Biomass / Calcium / Cell Adhesion / Atomic Force Microscopy / Multidisciplinary / Computer Simulation / Hydrogen Bonding / Protein Conformation / Protein Binding / Normal Distribution / Site-directed Mutagenesis / Ligands / Ions / Nature Communications / Calcium / Cell Adhesion / Atomic Force Microscopy / Multidisciplinary / Computer Simulation / Hydrogen Bonding / Protein Conformation / Protein Binding / Normal Distribution / Site-directed Mutagenesis / Ligands / Ions / Nature Communications

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Improving Protein Pharmacokinetics by Genetic Fusion to Simple Amino Acid Sequences

Biotechnology / Biological Chemistry / Biological Sciences / Liver / Mice / Animals / Epitope mapping / Proline / Proteins / Glycoproteins / Neuraminidase / CHEMICAL SCIENCES / Rats / Time Factors / Amino Acid Sequence / Recombinant Proteins / Protein Binding / Site-directed Mutagenesis / Molecular Sequence Data / Animals / Epitope mapping / Proline / Proteins / Glycoproteins / Neuraminidase / CHEMICAL SCIENCES / Rats / Time Factors / Amino Acid Sequence / Recombinant Proteins / Protein Binding / Site-directed Mutagenesis / Molecular Sequence Data

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Engineering of Pyridine Nucleotide Specificity of Nitrate Reductase: Mutagenesis of Recombinant CytochromebReductase Fragment ofNeurospora crassaNADPH:Nitrate Reductase

Kinetics / Protein Engineering / Neurospora crassa / Escherichia coli / Nitrate Reductase / Enzyme / Steady state / Substrate Specificity / Amino Acid Profile / Amino Acid Sequence / Base Sequence / Recombinant Proteins / Site-directed Mutagenesis / Binding Site / Nucleotides / Biochemistry and cell biology / Molecular Sequence Data / Enzyme / Steady state / Substrate Specificity / Amino Acid Profile / Amino Acid Sequence / Base Sequence / Recombinant Proteins / Site-directed Mutagenesis / Binding Site / Nucleotides / Biochemistry and cell biology / Molecular Sequence Data

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Human Mitochondrial Manganese Superoxide Dismutase Polymorphic Variant Ile58Thr Reduces Activity by Destabilizing the Tetrameric Interface † , ‡

Biochemistry / Polymorphism / Electrochemistry / Oxidative Stress / Amyotrophic Lateral Sclerosis / Free Radical / Crystal structure / Humans / Temperature / Superoxide Dismutase / Phenotype / Enzyme / Body Temperature / Temperature Dependence / Active site / Thermal Stability / Oxidative Damage / Genetic variation / Enzyme activity / Protein Conformation / Elevated Temperature / Site-directed Mutagenesis / Nervous System Diseases / Biochemistry and cell biology / Molecular Structure / Melting Temperature / Molecular Sequence Data / Free Radical / Crystal structure / Humans / Temperature / Superoxide Dismutase / Phenotype / Enzyme / Body Temperature / Temperature Dependence / Active site / Thermal Stability / Oxidative Damage / Genetic variation / Enzyme activity / Protein Conformation / Elevated Temperature / Site-directed Mutagenesis / Nervous System Diseases / Biochemistry and cell biology / Molecular Structure / Melting Temperature / Molecular Sequence Data

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