TLC-Densitometric Quantification of Negundoside, Ursolic Acid, Eugenol, Lupeol and Β;Sitosterol Using HPTLC from Vitex Negundo Leaves

Vitex negundo Linn (Verberaceae) is an important drug in Indian, Chinese and Malaysian Systems of medicine. Leaves are reported to show anti-inflammatory, anti-ulcer, anti-implantation and analgesic activities. They contain negundoside, nishindaside, agnuside, luteolin, vitexicarpin, ursolic acid and β-sitosterol.Lupeol, an important marker compound has not been reported from V. negundo till date. Our preliminary TLC experiments indicated the presence of lupeol