Nucleotide sequence of porcine preprolactin cDNA

Nucleic Acids Research

Volume 17 Number 8 1989

Nucleotide sequence of porcine preprolactin cDNA Schulz-Aellen*, Elizabeth Schmid* and Rao N.Movva+

Marie-Franqoise

Biogen SA Submitted March 16, 1989

EMBL accession no. X14068

The DNA complementary to the mRNA encoding porcine prolactin (pPrl) was cloned in E. coli. The complete nucleotide sequence of 891 bp cDNA was determined. The cDNA encodes a protein precursor comprising a 30 amino acid signal peptide followed by a mature Prl sequence of 199 amino acids. The cDNA is shown to include a 3' untranslated region of 142 bp containing the consensus AAUAAA sequence. Comparison of the amino acid sequence of the mature pPrl deduced from the nucleotide sequence with the previously published pPrl amino acid sequence (1) shows a single amino acid difference at position 13; methionine is identified instead Furthermore, the nucleotide sequence estabof the reported valine. lishes the presence of a glutamine and an asparic acid at position 122 and 196 respectively. The mRNA and protein sequences of pPrl were compared to the corresponding Prl sequences obtained in rat, bovine and human (2, 3, 4) and show a greater nucleotide than amino acid homology in each case. Their signal sequences show less homology than that observed with their mature Prl sequences. Finally, the same degree of preference for G or C in codon third positions is observed in pPrl as in all members of the prolactin growth hormone set of genes. ATCACCGCCATGGACAACACAGGGTCGTCACAGAAAGGGTCACTCCTGCTCCTACTGCTGCTGGTGTCAAATCTGTTCTTGTGCAAGAGCGTGGCCTCC M T

N D

G

5 5

Q

K

S

G

L

L

L

L

L

L

L

V

SI

-20

-30

L

F

L

C

K

S

V

A

CTGCCCATCTGCCCCAGCGGGGCTGTCAACTGCCAGATGTCCCTTCGAGACCTGTTCGACCGTGCAGTCATCCTGTCCCACTACATCCATAACCTCTCT L +1

P

I

C

P S

G

A

V

I

C

QG

S

L

R

D

L

F

10

D

R

A

V

99

S

-1

-10 I L

SIT

II *

I

L

190

S

30

20

TCGGAAATGTTCAACGAATTTGATAAAAGGTATGCCCAGGGCAGAGGGTTCATTACCAAGGCCATCAACAGCTGCCACACCTCCTCCCTCTCTACGCCT S T A Q G R G FIT K A I N S C I T S S L IS P S E M F N E F D K 40 60 S5

297

GAAGACAAAGAGCAAGCCCAACAGATCCATCATGAAGTCCTCCTGAACTTGATACTGAGAGTGCTGCGCTCCTGGAATGACCCGCTGTATCACCTGGTC 396 e

D

K

E

Q

AG

Q

I

1 1 K

V

L

L I

L

I L

R

V

L

V W

R

s5

70

I

D

P

L

T I

L

V

90

ACGGAAGTGAGGGGTATGCAGGAAGCCCCAGATGCTATCCTCTCGAGAGCCATAGAGATCGAGGAACAAAACAAACGGCTTCTAGAAGGCATGGAGAAG 495 T

E

V

R

G M

S A

Q

P

100

D

A

I

L

S

R

A

I

S

K

I

Q

I X

R

L

L I

G M

K

130

120

110

ATAGTCGGCCAGGTCCATCCTGGAATCAAGGAGAATGAGGTCTACTCTGTGTGGTCCGGACTTCCCTCCCTGCAGATGGCTGATGAAGACACTCGCCTT 594 I V

G

Q

V

H

G

P

IK K

N

S

V

T

S

V

140

W

S

G

L

P

S

L

QG

150

A

D 160

I

D

T I

L

TTTGCTTTTTATAACCTGCTCCACTGCCTACGCAGGGATTCACATAAGATTGACAATTATCTCAAGCTTCTCAAGTGCCGAATCATCTACGACAGCAAC 093 F

A

F

YT

L

170

L E

C

L

R

R

D

S

K

I

D I

T

L

100

K

L

C L I 190

R1I

T

D I I

TGCTAAGCCCACGTCCATCCCGTCTGTTTCTTAACTGTTTCTTAACGCTCCATCCCATAGAAAGATTCTTTTAGTTTTATAGCTTTTTAATGCATGCTT 792 C TER 199 TCC GTAGAG ATGTCA GGGTG TAACGCATCT CCTCTTAAAAAATAAGAC

091

Present addresses:*Glaxo IMB, 46 Route des Acacias, 1227 Carouge, Geneva and +Sandoz AG, 4002 Basel, Switzerland

References 1) Li, C.H. (1976) Int. J. Peptide Protein Res. 8:205-224 2) Cooke, N.E. et al (1980) J. Biol. Chem. 255:6502-6510 3) Miller, W.L. et al (1981) DNA 1:37-50 4) Trong, A.T. et al (1984) EMBO J. 3:429-437

© I R L Press

3295