Novel peptide recognized by RhoA GTPase

May 28, 2017 | Autor: Jacek Otlewski | Categoría: Fluorescence Spectroscopy, Kinetics, Epitope mapping, Phage display, Peptides, Guanosine Triphosphate, Protein Conformation, Amino Acid Sequence, Recombinant Proteins, Nucleotides, Biochemistry and cell biology, Guanosine Triphosphate, Protein Conformation, Amino Acid Sequence, Recombinant Proteins, Nucleotides, Biochemistry and cell biology

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A phage-displayed random 7-mer disulfide bridge-constrained peptide library was used to map the surface of the RhoA GTPase and to find peptides able to recognize RhoA switch regions. Several peptide sequences were selected after four rounds of enrichment, giving a high signal in ELISA against RhoA-GDP. A detailed analysis of one such selected peptide, called R2 (CWSFP- GYAC), is reported.

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Novel peptide recognized by RhoA GTPase

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