In vitro anthelmintic activity of Sansevieria trifasciata leaves extract against Fasciola hepatica

May 26, 2017 | Autor: W. Pharmaceutical... | Categoría: Pharmacology, Biochemistry, Pharmacy, Toxicology
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The purpose of this study was to investigate the in vitro anthelmintic activity of Sansevieria trifasciata leaves extract against Fasciola Hepatica. The extract was prepared by soaking one portion of the plant leaves into absolute ethanol and another portion in distilled water for 3 days. The extracted plant material was then squeezed through a piece of porous cloth. The extracts were concentrated in a rotary evaporator at 40°C under reduced pressure and later stored at 4°C until use. The flukes were collected and transferred in flasks containing normal saline maintained at a temperature of 37 0 C to the laboratory in school of health Science, Mount Kenya University for experimental protocol. S. trifasiata leaves tested positive for flavanoids, tannins, alkaloids, phenols, steroids and saponins. The in-vitro assay showed that different doses of the extract resulted to death of the parasites at different mean time.
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