Journal of Neuroimmunology 162 (2005) 194 – 196 www.elsevier.com/locate/jneuroim
Erratum
Erratum to bGangliosides act as onconeural antigens in paraneoplastic neuropathiesQ [J. Neuroimmunol. 156 (2004) 178–187] Luca De Tonia,1, Silvia Marconia,1, Ettore Nardellia,2, Daniela Albertia, Giovanna Borsellinob, Giulio Fracassoc, Simona Bachb, Laura Bertolasia, Antonio Santod, Antonella Bassie, Daniela Tramontib, Luca Battistinib, Bruno Bonettia,T a
Institute of Neurology, Azienda Ospedaliera of Verona, P. Scuro, 37134 Verona, Italy b Laboratory of Neuroimmunology, I.R.C.C.S. Santa Lucia, Roma, Italy c Institute of Immunology, Azienda Ospedaliera of Verona, 37134 Verona, Italy d Institute of Oncology, Azienda Ospedaliera of Verona, 37134 Verona, Italy e Institute of Clinical Chemistry, Azienda Ospedaliera of Verona, 37134 Verona, Italy
The Publisher regrets that Figs. 1 and 4 of the above article were incorrectly printed in black and white. The figures have been reproduced in colour below. We sincerely apologise for any inconvenience caused to those concerned.
DOI of original article 10.1016/j.jneuroim.2004.07.020. T Corresponding author. Tel.: +39 45 8074285; fax: +39 45 585933. E-mail address:
[email protected] (B. Bonetti). 1 These authors equally contributed to the work. 2 Present address: U.O. of Neurology, San Bonifacio Hospital, 37047 Verona, Italy. 0165-5728/$ - see front matter D 2005 Elsevier B.V. All rights reserved. doi:10.1016/j.jneuroim.2005.01.012
L. De Toni et al. / Journal of Neuroimmunology 162 (2005) 194–196
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Fig. 1. Expression of gangliosides on lung adenocarcinoma. Immunohistochemistry on carcinoma from neoplastic controls showed faint signals for GM1 (A) and more pronounced reactivity for GD3 (B). On adenocarcinoma from patient 1, the majority of neoplastic cells displayed high levels of GM1 (C), GD1a (D) and GD3 (E); immunoreactivity for GM3 (F) and GM2 (G) was detectable with lower intensity, while GD1b (H) and GQ1b (I) were not expressed. Peroxidase, hematoxylin counterstain. Scale bars: A, B, H, I=50 Am; C, E–G=100 Am; D=150 Am.
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L. De Toni et al. / Journal of Neuroimmunology 162 (2005) 194–196
Fig. 4. IgG from patient 1 recognized lipid autoantigens in normal neural tissue and in his tumor. Biotinylated IgG from patient 1 (A), but not from healthy controls (B), immunostained motor neurons in human spinal cord and neurons of dorsal root ganglia (C). Double immunoflourescence for GM1 (D and G) and IgG from patient 1 (E and H) showed a partial co-localization on Schwann cells and myelin sheaths in posterior (F) and anterior (I) roots (resulting in orange signals) but not on axons, which were recognized only by patient 1’s IgG and appeared red. Double staining for GDIa (J) and IgG from patient 1 (K) showed co-localization (L) in anterior roots on focal structures morphologically resembling nodes of Ranvier (arrows). Biotinylated IgG from patient 1 (M), but not from healthy controls (N), immunostained his neoplastic cells and the signal was abolished after extraction of glycolipid antigens (O). In the autoptic nervous tissue of patient 1, double staining for S-100 (P) and IgG (Q) showed co-localization on Schwann cells (orange signals), but not on axons, which were positive only for IgG (R). Scale bars: A–C=150 Am; D–I, P–R=100 Am; J–L=200 Am.
