Effect of intrathoracic pressure on plasma arginine vasopressin levels (pAVP)

P4 EXPRESSION OF A NOVEL T CELL ACTIVATION ANT"CSN 508+ T LYMPHOCYTES IN VIRAL CHRONIC ACTIVE HEPATITIS.

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Garcia-pnz6”f,R.

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INTRAHEPATIC

f%jares*.Garcfa-sfinohez’~,g., A*

Lbpez-Sotet , M. 0. de #a"dAzuri and F. SQnchez-Madrid . Castroe"tarol"gy Pathology* and Immunology Services. Hospital de la Princesa. Madrid. Spain.

liver cell i&y in viral chronic %ive-hepatitis (CAti). However. the mere prekce of T lymphocytes with cytoto%ic/suppressor phenotype in the hepatic icflammatory infiltrate does reaction. I" the present study. we have investieeted the expression oi different activation antigens s"fb as the IL-PR. the W-R and a recently described T cell ectivatio" molecule termed AI" 0" the different lymphocyte subsets of both peripheral blood and liver-infiltrating lymphocytes n-om patients with viral CAH. Studies of double inmunostaining clearly shoved that the ..+rjority of intpahepatic CDS+ T lymphocytes selectively coexpressed the AIN antigen. whereas no significant expression of this molecule was found on either intrabepatic CD& T lymphocytes or on peripheral blood lymphocytes of the same patients. By contrast. few and scattered liver-infiltrating AINc cells were observed in patients with chronic persistent hepatitis. Furthermore, lymphocytes from bpth peripheral blood or liver compartments did not express other activation ant&ens such as the IL-ZR and the W-R moLcc"les. These results sw?Sest that this COB+ AIM+ T cell subset may represent the main activated intrahepatic lymphoid cell population in viral CAH. u"derli"i"S the major role of this T cell subset in the pathogenesis of hepatocellular damage in this liver disease.

nit-nec;ssarily imply that these celle ere aktively involved in en inm""e

patients with decorpemsated liver cirrhosis, particularly those with h~natremia. often have elevated PAW. Di.ioisbed 'effective. blood rolum? my stilllate rm,,,smtiC release of AW. lntretborecic "rJl"l@ receptoie are aer.?.itiveto the atria1 trrmemJra1 press"re gradient (ATpG,. Raised intrathomoio presssure (ITP) -, reduce ATF-3 and a~ be a factor favoring AW release. Abdomtml distension is acampanied by an elevation of diapbrqm and a rise in ITP. Studies from our labaratory have sbom that abdaniml distensi~ll during paeuloperitoneum WV) results in a prom@ and arked in0reae-e in PAW. Ye bra studied the effect of PI (20 petients) arId peraoentesis wC) ml decmpensated cirrhotic patients, on pAVP (MA. Biihlan Lab, Besel). ITP, right atria1 pressure iFtAP,and ATPO. &sI&&: 1, pAVP increased ei+ffcantly durirq PI (16fl6.6 pelti; p