Appl Microbiol Biotechnol (2001) 57:514 DOI 10.1007/s002530100799
E R R AT U M
V. Pauliina Lankinen · Alice M. Bonnen Lori H. Anton · David A. Wood · Nisse Kalkkinen Annele Hatakka · Christopher F. Thurston
Characteristics and N-terminal amino acid sequence of manganese peroxidase from solid substrate cultures of Agaricus bisporus Published online: 30 August 2001 © Springer-Verlag 2001
Appl Microbiol Biotechnol (2001) 55:170–176 Due to a technical error, on page 173 of the print version, the row of units in the table header of table 1 was misplaced. Table 1 is reproduced correctly here. Table 1 Yield of manganese peroxidase from a purification of proteins from Agaricus bisporus compost extract. Each unit (U) of enzyme activity is equivalent to 1 µmol of product min–1 Sample
Vol (ml)
Protein (mg ml–1)
Activity (U ml–1)
Total activity (U)
Recovery (%)
Compost extract I Anion exchange Sepharose Q (batch) II Anion exchange Sepharose Q I Gel filtration Sephacryl S-200 II Gel filtration Sephacryl S-200
1460.0 118.0 2.3 0.7 1.2
–a –a 8.5 10.5 4.8
0.1 1.1 36.7 148.6 14.0
175.2 129.8 84.4 104.0 16.8
100 74 48 59 10
Specific activity (U mg–1)
4.3 9.9 3.5
a The brown coloured material in the compost extract made estimation of protein impossible, preventing the description of specific activity
The online version of the original article can be found at http://dx.doi.org/10.1007/s002530000509 V.P. Lankinen · A. Hatakka (✉) Department of Applied Chemistry and Microbiology, Biocenter 1, Viikinkaari 9, P.O. Box 56, 00014 University of Helsinki, Finland e-mail:
[email protected] Tel.: +358-9-19159314, Fax: +358-9-19159322 A.M. Bonnen 63 Carrisbrooke Lane, Winston-Salem, NC 27104, USA L.H. Anton 1001 Cambria Avenue, Windfer, PA 15963, USA D.A. Wood Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1, Inohana, Chuo-Ku, Chiba 260, Japan N. Kalkkinen Institute of Biotechnology, Biocenter 1, Viikinkaari 9, P.O. Box 56, 00014 University of Helsinki, Finland C.F. Thurston Microbiology Group, Division of Life Sciences, King’s College London, Campden Hill Road, London W8 7AH, UK
