Biologically Localized Firefly Luciferase: A Tool to Study Cellular Processes

evidence that the transfer of intermediates (I) between two soluble en-

through allosteric cooperative effects between the association site and the

tion of coumarate by ATP,

concentrated immobilized PK accumulates rapidly inside the beads before

lowing.

3913-3919. Copyright 1987 American Chemical Society.

b. Internal diffusion. When an enzyme is embedded in a porous matrix, the substrate is also, in this case, subject to two concurrent processes:

the rate of disappearance of substrate (at the interface) from the medium.

The light output by luciferase in these conditions is linearily related to

atractyloside, which blocks the transport of nucleotides through the mito-

surface of mitochondria.

colonies or plaques expressing luciferase. Moreover, the film darkening

the straightforward use of luciferase as a

Gould, S. J., Krisans, S., Keller, G.-A., and Subramani, S (1990b). J. Cell Biol. 110. 27-34.

and L. Packer, eds.), Vol. 56, pp. 530-544.