Amino acid sequence of ammodytoxin C as deduced from cDNA

Nucleic Acids Research

Volume 17 Number 11 1989

Amino acid sequence of ammodytoxin C as deduced from cDNA Joze Punyger6ar, Dusan Kordig, Roman Jerala, Mojca Trstenjak-Prebanda, Marko Dolinar, Vladka Curin-Serbec, Rado Komell and Franc Gubensek Department of Biochemistry, J.Stefan Institute and lInstitute of Biochemistry, Medical Faculty, E.Kardelj University, 61000 Ljubljana, Yugoslavia EMBL accession no. X15138 Submitted May 12, 1989

Ammodytoxins A, B and C are presynaptically toxic phospholipases A isolated from the venom of the European viper Vipera ammodytes aLmodytes (1-3). A venom gland eDNA library was constructed in pUC9 from the snakes collected around Slunj, Croatia. The cDNA sequence encoding ammodytoxin C shows that a 122 residue long mature toxin is synthesized with a 16 residue long leading peptide, which is 81% similar to the leading peptides of the crotoxin A and B subunits from Crotalus durissus terrificus (4), whereas the protein sequence similarity with the mature crotoxin B subunit is only 59%. The Elapidae and Hydrophiidae toxic phospholipases A2 belong to another subgroup of enzymes and have longer leading peptides (cf. 5).

CAGAGGGAGCCTGGAGGTGCTTCTGGACCCCCTTCAACTCTGAGACAAGGCTGCCAGCTGTCTGGATTCAGGAGG 75 ATGAGGACTCTCTGGATAGTGGCCGTGTGCCTGATAGGCGTCGAGGGGAGCCTGCTCGAATTCGGGATGATGATC 150 M

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-16

A -10

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G S -1 +1

L

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M

M

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CTGGGGGAGACGGGGAAAAACCCTCTTACCTCCTACTCCTTTTACGGATGCTACTGCGGCGTGGGGGGGAAAGGC 225 L G E T G K N P L T S Y S F Y G C Y C G V G G K G ACGCCAAAGGACGCCACCGACCGCTGCTGCTTCGTGCATGACTGCTGTTATGGGAATCTGCCTGACTGCAGCCCC 300 T P K D A T D R C C F V H D C C Y G N L P D C S P AAAACGGACAGATACAAATACCACCGGGAGAACGGAGCTATCGTCTGTGGAAAAGGCACCTCGTGCGAGAATCGG 450 R K

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C

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ATTTGTGAGTGTGACAGGGCCGCGGCAATCTGCTTCCGAAAGAATCTGAAGACATACAACTATATATATAGGAAT 500 I C E C D R A A A I C F R K N L K T Y N Y I Y R N TACCCAGACATTTTGTGCAAGGAGGAGTCAGAGAAATGCTAAGTCTCTGCAGGCCGGGAAAAAACCCTCCAATTA 575 C * 122

CACAATCGTAGTTGTGTTACTCTATTATTCTGAATGCAATACTGAGTAATAAACAGGTGCCAGCTTTGCACTCAA AAAAAAA

600 607

Acknowledgements This work was supported by Research Community of Slovenia and by Federal Secretariat for Development. References: 1.Ritonja A. and Gubensek F. (1985) Biochim. Biophys. Acta, 828,

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2.Ritonja A., Machleidt W., Turk V. and Gubensek F.(1986) Biol. Chem. Hoppe-Seiler, 367, 919 3.Krizaj I.,Turk D.,Ritonja A. and Gubensek F. (1989) unpublished 4.Buchier C., Ducancel F., Guignery-Frelat G.,Bon C., Boulain J.C. and Menez A. (1988) Nucleic Acid Res., 16, 9050 5.Ducancel F. Guignery-Frelat G., Bouchier C., Menez A. and Boulain J.C. (1988) Nucleic Acid Res., 16, 9049

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