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A New Era of Molecular Breeding Using Next-generation Sequencing
Introduction Molecular breeding applies molecular biology tools to accelerate the breeding process. One of the most important methods of molecular breeding is marker assisted selection (MAS), the use of DNA markers that are tightly-linked to target loci as a substitute to assist phenotypic screening. Next-generation sequencing (NGS) can decode a whole genome or transcriptome in a very short time and is the most powerful tool to identify and develop DNA/RNA molecular markers for MAS. As the largest genomics center in the world, BGI-tech has developed new services to advance the applications of NGS technology in the molecular breeding area. With our services, scientists can complete their breeding projects in one year, which would have otherwise taken six to eight years using traditional strategies. Our approach provides dramatic savings of time and costs.
Research strategy
Genome
De novo assembly
Variation map construction
Re-sequencing, RAD, GBS, Microarray
Functional gene identification
Breeding promotion
Mutation detection and selection
Demo case Case 1 Drought resistant foxtail millet Genome sequence of foxtail millet (Setaria italica) provides insights into grass evolution and biofuel potential. Zhang G et. al. Nat Biotechnol. 2012. Background Foxtail millet（Setaria italic Beauv）is one of the traditional dominant cereal crops with high nutritional value in the arid and semi-arid regions of north China. It would be very beneficial to be able to improve its yield potential, grain quality, and stress tolerance by utilizing heterosis. Due to self-pollination and small-vast spikelet, it is difficult to breed millet. One of the key ways to make use of the hybrid vigor in millet is the breeding and selection of elite male-sterile lines. An excellent male-sterile line has great potential for subsequent breeding.
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Research strategy a). 'Zhanggu'×A2 (population construction)
e). Construct a genetic map
b). Whole Genome Shotgun
f ). Identify variations
c). Assemble a draft genome of ‘Zhanggu’
g). 7 genes detection
d). Re-sequence A2
Comparison between conventional and MAS backcrossing
Genome map and linkage map of Foxtail millet.
Results Through the use of whole-genome molecular breeding, we successfully improved A2 to green A2. Interestingly, we also obtained two types of one new male-sterile A2 line: short growth phase and long growth phase A2. They can be appropriate for different growing regions.
Case 2 Cucumis melon breeding Background Melon (Cucurbitaceae cucumis) is an annual herb widely cultivated worldwide as an important agricultural crop. China has the largest cultivation region and the highest yield in the world. Although the numerous varieties of the melon have been developed through traditional crossbreeding, there are still shortcomings and deficiencies. While maintaining the yield of hybrid and wide adaptability traits, we directionally improved other traits including background peel, flesh color, and so forth. We help the scientist generated several new varieties that combine the excellent traits of the parents. Research strategy and results
High density molecular markers of melon
We obtain the improved variety with yellow rind, thick crazing, yellow pulp and higher yield by using NGS based molecular breeding. +86-(0)755-25273045
[email protected]
www.bgitechsolutions.com
