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Transfection
Transfection
Transient expression and insertional mutagenesis of Puccinia triticina using biolistics
Genetics / Molecular Biology / Gene expression / Sequence Analysis / Helium / Pressure / Gene Function / Heat Shock / Fungal genome size / Transfection / Base Sequence / Wheat Leaf Rust / BASIDIOMYCOTA / Transient Expression / Molecular Sequence Data / Pressure / Gene Function / Heat Shock / Fungal genome size / Transfection / Base Sequence / Wheat Leaf Rust / BASIDIOMYCOTA / Transient Expression / Molecular Sequence Data
Caracterización funcional de la podocalicina humana expresada en sistemas heterólogos
Fluorescence Microscopy / Cell Biology / Transmission Electron Microscopy / Mammalian Cell Culture / Genetic Engineering / Green Fluorescent Protein / Transfection / Heterologous Expression / Podocalyxin / Podocalicina humana / Green Fluorescent Protein / Transfection / Heterologous Expression / Podocalyxin / Podocalicina humana
Nonbilayer Phase of Lipoplex–Membrane Mixture Determines Endosomal Escape of Genetic Cargo and Transfection Efficiency
Genetics / Technology / Atomic Force Microscopy / Biological Sciences / DNA / Molecular / Lipids / Cercopithecus aethiops / Animals / Therapy / Osmotic pressure / Liposomes / Plasmids / Gene Delivery / X ray diffraction / Transfection / Lipid bilayers / Molecular Targeted Therapy / Endosomes / Nucleic Acid / Molecular / Lipids / Cercopithecus aethiops / Animals / Therapy / Osmotic pressure / Liposomes / Plasmids / Gene Delivery / X ray diffraction / Transfection / Lipid bilayers / Molecular Targeted Therapy / Endosomes / Nucleic Acid
Functional characterization of alternatively spliced human SECISBP2 transcript variants
Molecular Evolution / Neurochemistry / Immunohistochemistry / Biological Sciences / Phylogeny / Cell line / Environmental Sciences / Humans / Kidney / Ultraviolet / Animals / Muscles / Alternative splicing / Nucleic Acids / Serotonin Receptors / RNA-binding proteins / Insertion sequence / Antisense Oligonucleotide Drugs / Amino Acid Profile / Transfection / Alternative Splicing / Protein isoforms / Amino Acid Sequence / In Silico / Protein Binding / Neurosciences / Full Length Movies / Protein Biosynthesis / Gene Expression Regulation / Mitochondrial Proteins / Molecular Sequence Data / Cell Membrane / Cell line / Environmental Sciences / Humans / Kidney / Ultraviolet / Animals / Muscles / Alternative splicing / Nucleic Acids / Serotonin Receptors / RNA-binding proteins / Insertion sequence / Antisense Oligonucleotide Drugs / Amino Acid Profile / Transfection / Alternative Splicing / Protein isoforms / Amino Acid Sequence / In Silico / Protein Binding / Neurosciences / Full Length Movies / Protein Biosynthesis / Gene Expression Regulation / Mitochondrial Proteins / Molecular Sequence Data / Cell Membrane
Gene transfer efficiency of high primary amine content, hydrophobic, alkyl-oligoamine derivatives of polyethylenimine
Biomaterials / Nanoparticle / Multidisciplinary / DNA / Cell line / Humans / Amines / Mice / Animals / Gene transfer techniques / Dynamic Light Scattering / Plasmids / Particle Size / Carboxylic Acids / Transfection / Polyethylenimine / Plasmid DNA / Polyethyleneimine / Gene Transfer / Molecular Structure / Ethidium bromide / Humans / Amines / Mice / Animals / Gene transfer techniques / Dynamic Light Scattering / Plasmids / Particle Size / Carboxylic Acids / Transfection / Polyethylenimine / Plasmid DNA / Polyethyleneimine / Gene Transfer / Molecular Structure / Ethidium bromide
Identification of amino acid residues critical for endonuclease and integration activities of HIV-1 IN protein in Vitro
Bacteriology / Catalysis / Flow Cytometry / Virology / Calcium / Biological Chemistry / Magnetic Resonance Spectroscopy / Gene expression / Cell separation / Biocatalysis / Signal Transduction / HIV / Biological Sciences / Cell line / Identification / Humans / Mutagenesis / Sequence alignment / Mutation / Placenta / Virus / Escherichia coli / Animals / Plants / Monoclonal Antibodies / Chemotaxis / Phosphorylation / Plasmids / Affinity chromatography / Phenotype / Phosphotyrosine / CHEMICAL SCIENCES / Molecular cloning / Amino Acids / Mycoplasma Genitalium / Lysine / Amino Acid Profile / Transfection / Protein Conformation / Amino Acid Sequence / Base Sequence / Amino Acid Substitution Rates / Chemokines / Structure activity Relationship / Newcastle Disease Virus / Recombinant Proteins / Protein Binding / Hydrolysis / DNA binding proteins / Site-directed Mutagenesis / Interleukin / Interferon Alpha / Down-Regulation / Molecular Sequence Data / Biological Chemistry / Magnetic Resonance Spectroscopy / Gene expression / Cell separation / Biocatalysis / Signal Transduction / HIV / Biological Sciences / Cell line / Identification / Humans / Mutagenesis / Sequence alignment / Mutation / Placenta / Virus / Escherichia coli / Animals / Plants / Monoclonal Antibodies / Chemotaxis / Phosphorylation / Plasmids / Affinity chromatography / Phenotype / Phosphotyrosine / CHEMICAL SCIENCES / Molecular cloning / Amino Acids / Mycoplasma Genitalium / Lysine / Amino Acid Profile / Transfection / Protein Conformation / Amino Acid Sequence / Base Sequence / Amino Acid Substitution Rates / Chemokines / Structure activity Relationship / Newcastle Disease Virus / Recombinant Proteins / Protein Binding / Hydrolysis / DNA binding proteins / Site-directed Mutagenesis / Interleukin / Interferon Alpha / Down-Regulation / Molecular Sequence Data
Identification of amino acid residues critical for endonuclease and integration activities of HIV1 IN protein in Vitro
Bacteriology / Catalysis / Flow Cytometry / Virology / Calcium / Biological Chemistry / Magnetic Resonance Spectroscopy / Gene expression / Cell separation / Biocatalysis / Signal Transduction / HIV / Biological Sciences / Cell line / Identification / Humans / Mutagenesis / Sequence alignment / Mutation / Placenta / Virus / Escherichia coli / Animals / Biological / Plants / Monoclonal Antibodies / Chemotaxis / Phosphorylation / Plasmids / Affinity chromatography / Phenotype / Phosphotyrosine / CHEMICAL SCIENCES / Molecular cloning / Amino Acids / Mycoplasma Genitalium / Lysine / Amino Acid Profile / Transfection / Protein Conformation / Amino Acid Sequence / Base Sequence / Amino Acid Substitution Rates / Chemokines / Structure activity Relationship / Newcastle Disease Virus / Recombinant Proteins / Protein Binding / Hydrolysis / DNA binding proteins / Site-directed Mutagenesis / Interleukin / Interferon Alpha / Down-Regulation / Molecular Sequence Data / Biological Chemistry / Magnetic Resonance Spectroscopy / Gene expression / Cell separation / Biocatalysis / Signal Transduction / HIV / Biological Sciences / Cell line / Identification / Humans / Mutagenesis / Sequence alignment / Mutation / Placenta / Virus / Escherichia coli / Animals / Biological / Plants / Monoclonal Antibodies / Chemotaxis / Phosphorylation / Plasmids / Affinity chromatography / Phenotype / Phosphotyrosine / CHEMICAL SCIENCES / Molecular cloning / Amino Acids / Mycoplasma Genitalium / Lysine / Amino Acid Profile / Transfection / Protein Conformation / Amino Acid Sequence / Base Sequence / Amino Acid Substitution Rates / Chemokines / Structure activity Relationship / Newcastle Disease Virus / Recombinant Proteins / Protein Binding / Hydrolysis / DNA binding proteins / Site-directed Mutagenesis / Interleukin / Interferon Alpha / Down-Regulation / Molecular Sequence Data
Imaging Erg and Jun transcription factor interaction in living cells using fluorescence resonance energy transfer analyses
Fluorescence Microscopy / Transcription Regulation / Confocal Microscopy / Gene expression / Fluorescence Resonance Energy Transfer / Western blotting / Humans / FRET / Transcriptional regulation / Plasmids / Protein Interactions / Transcription Factor / Physical Interaction / Cell nucleus / Protein Interaction / HeLa cells / Transfection / In Vitro Studies / Protein Binding / Flim / Fusion Protein / DNA binding proteins / Fluorescence Lifetime Imaging / Biochemistry and cell biology / Dimerization / Transient Expression / Western blotting / Humans / FRET / Transcriptional regulation / Plasmids / Protein Interactions / Transcription Factor / Physical Interaction / Cell nucleus / Protein Interaction / HeLa cells / Transfection / In Vitro Studies / Protein Binding / Flim / Fusion Protein / DNA binding proteins / Fluorescence Lifetime Imaging / Biochemistry and cell biology / Dimerization / Transient Expression
Imaging Erg and Jun transcription factor interaction in living cells using fluorescence resonance energy transfer analyses
Fluorescence Microscopy / Transcription Regulation / Confocal Microscopy / Gene expression / Fluorescence Resonance Energy Transfer / Western blotting / Humans / FRET / Transcriptional regulation / Plasmids / Protein Interactions / Transcription Factor / Physical Interaction / Cell nucleus / Protein Interaction / HeLa cells / Transfection / In Vitro Studies / Protein Binding / Flim / Fusion Protein / DNA binding proteins / Fluorescence Lifetime Imaging / Biochemistry and cell biology / Dimerization / Transient Expression / Western blotting / Humans / FRET / Transcriptional regulation / Plasmids / Protein Interactions / Transcription Factor / Physical Interaction / Cell nucleus / Protein Interaction / HeLa cells / Transfection / In Vitro Studies / Protein Binding / Flim / Fusion Protein / DNA binding proteins / Fluorescence Lifetime Imaging / Biochemistry and cell biology / Dimerization / Transient Expression
Local RNA Target Structure Influences siRNA Efficacy: A Systematic Global Analysis
Thermodynamics / Molecular Biology / Computational Biology / Gene Silencing / Global Analysis / Genetic Engineering / Molecular / RNA interference / Cercopithecus aethiops / Cell line / Humans / RNAi / Animals / Drug Design / Free Energy / Rats / dsRNA / Substrate Specificity / Fluorescent Protein / Transfection / siRNA / RNA Structure / Base Sequence / TRP / UTR / GFP / Nucleic Acid Conformation / Biochemistry and cell biology / Enterovirus / Genetic Engineering / Molecular / RNA interference / Cercopithecus aethiops / Cell line / Humans / RNAi / Animals / Drug Design / Free Energy / Rats / dsRNA / Substrate Specificity / Fluorescent Protein / Transfection / siRNA / RNA Structure / Base Sequence / TRP / UTR / GFP / Nucleic Acid Conformation / Biochemistry and cell biology / Enterovirus
Local RNA Target Structure Influences siRNA Efficacy: A Systematic Global Analysis
Thermodynamics / Molecular Biology / Computational Biology / Gene Silencing / Global Analysis / Genetic Engineering / Molecular / RNA interference / Cercopithecus aethiops / Cell line / Humans / RNAi / Animals / Drug Design / Free Energy / Rats / dsRNA / Substrate Specificity / Fluorescent Protein / Transfection / siRNA / RNA Structure / Base Sequence / TRP / UTR / GFP / Nucleic Acid Conformation / Biochemistry and cell biology / Enterovirus / Genetic Engineering / Molecular / RNA interference / Cercopithecus aethiops / Cell line / Humans / RNAi / Animals / Drug Design / Free Energy / Rats / dsRNA / Substrate Specificity / Fluorescent Protein / Transfection / siRNA / RNA Structure / Base Sequence / TRP / UTR / GFP / Nucleic Acid Conformation / Biochemistry and cell biology / Enterovirus
A Slow RNA Polymerase II Affects Alternative Splicing In Vivo
RNA / Biology / Drosophila melanogaster / Transcription Factors / Medicine / Biological Sciences / Humans / Animals / Hox Genes / Alternative splicing / Plasmids / Molecular Cell Biology / Enzyme / Time Factors / Transfection / Alternative Splicing / Protein isoforms / Embryos / Ribonucleases / Biological Sciences / Humans / Animals / Hox Genes / Alternative splicing / Plasmids / Molecular Cell Biology / Enzyme / Time Factors / Transfection / Alternative Splicing / Protein isoforms / Embryos / Ribonucleases
A Slow RNA Polymerase II Affects Alternative Splicing In Vivo
RNA / Biology / Drosophila melanogaster / Transcription Factors / Medicine / Biological Sciences / Humans / Animals / Hox Genes / Alternative splicing / Plasmids / Molecular Cell Biology / Enzyme / Time Factors / Transfection / Alternative Splicing / Protein isoforms / Embryos / Ribonucleases / Biological Sciences / Humans / Animals / Hox Genes / Alternative splicing / Plasmids / Molecular Cell Biology / Enzyme / Time Factors / Transfection / Alternative Splicing / Protein isoforms / Embryos / Ribonucleases
El arte de la mezcla
Genetics / Microbiology / Mycology / Molecular Biology / Plant Biology / Plasmids / Aspergillus Nidulans / Genetically Modified / Filamentous fungi / Fungal Genetics and Biology / Genetic Recombination / Transfection / Gene Disruption / Fungal Genetics / Genetic Selection / Plasmids / Aspergillus Nidulans / Genetically Modified / Filamentous fungi / Fungal Genetics and Biology / Genetic Recombination / Transfection / Gene Disruption / Fungal Genetics / Genetic Selection
An estrogen receptor chimera senses ligands by nuclear translocation
Biochemistry / Fluorescence Microscopy / Drug Discovery / Fluorescent Dyes and Reagents / STEROIDS / High Content Screening / Estrogen Receptor / Cercopithecus aethiops / Dexamethasone / Cell line / Humans / Mice / Animals / Immunoprecipitation / Tamoxifen / Time Factors / Transfection / Protein Binding / Cytoplasm / Ligands / Glucocorticoids / Estradiol / Protein Transport / Biochemistry and cell biology / High Content Screening / Estrogen Receptor / Cercopithecus aethiops / Dexamethasone / Cell line / Humans / Mice / Animals / Immunoprecipitation / Tamoxifen / Time Factors / Transfection / Protein Binding / Cytoplasm / Ligands / Glucocorticoids / Estradiol / Protein Transport / Biochemistry and cell biology
DNA damaging agents improve stable gene transfer efficiency in mammalian cells
Radiation / DNA damage / Cell line / Humans / Mice / Animals / Gene transfer techniques / Hydrogen Peroxide / Transfection / Recombination / Gene Transfer / Animals / Gene transfer techniques / Hydrogen Peroxide / Transfection / Recombination / Gene Transfer
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