Gene transfer techniques

SERCA2a Gene Transfer Enhances eNOS Expression and Activity in Endothelial Cells

Technology / Confocal Microscopy / Western blotting / Biological Sciences / Heart Failure / Cell line / Humans / Endothelial Cells / Congestive Heart Failure / Nitric oxide / Female / Animals / Gene transfer techniques / Endoplasmic Reticulum / Adeno-associated virus / Immunoprecipitation / Calcium Homeostasis / Endothelial Function / Endothelial cell / Swine / Protein Expression / Coronary Artery Disease / Vascular Reactivity / Molecular Targeted Therapy / Gene Transfer / Mitral regurgitation / Cell line / Humans / Endothelial Cells / Congestive Heart Failure / Nitric oxide / Female / Animals / Gene transfer techniques / Endoplasmic Reticulum / Adeno-associated virus / Immunoprecipitation / Calcium Homeostasis / Endothelial Function / Endothelial cell / Swine / Protein Expression / Coronary Artery Disease / Vascular Reactivity / Molecular Targeted Therapy / Gene Transfer / Mitral regurgitation

Lentiviral vector neutral endopeptidase gene transfer suppresses prostate cancer tumor growth

Prostate Cancer / Protein-Protein Interaction / Cell line / Humans / Mice / Animals / Gene transfer techniques / Male / Lentivirus / Cell Viability / Cancer Gene Therapy / Green Fluorescent Protein / Neoplasm Invasiveness / Protein Expression / Enzyme activity / Tumor Growth / Gene Transfer / Focal Adhesion Kinase / Animals / Gene transfer techniques / Male / Lentivirus / Cell Viability / Cancer Gene Therapy / Green Fluorescent Protein / Neoplasm Invasiveness / Protein Expression / Enzyme activity / Tumor Growth / Gene Transfer / Focal Adhesion Kinase

A comparative study on intra-articular versus systemic gene electrotransfer in experimental arthritis

Kinetics / Cytokines / Comparative Study / Gene expression / Collagen / Mice / Electroporation / Animals / Feasibility Studies / Gene transfer techniques / Male / Plasmids / Clinical Sciences / Cattle / Time Factors / Articular Cartilage Wear / Cell Proliferation / Knee Joint / Mice / Electroporation / Animals / Feasibility Studies / Gene transfer techniques / Male / Plasmids / Clinical Sciences / Cattle / Time Factors / Articular Cartilage Wear / Cell Proliferation / Knee Joint

Acquisition of an animal gene by microsporidian intracellular parasites

Biological Sciences / Animals / Gene transfer techniques / Host-parasite interactions / Microsporidia

Gene transfer in ovarian cancer cells: a comparison between retroviral and lentiviral vectors

Cancer / Flow Cytometry / Gene Therapy / Confocal Microscopy / Ovarian Cancer / Gene expression / Quantitative analysis / real time PCR / Humans / Female / Gene transfer techniques / Lentivirus / Quantitative Analysis / Transgene Expression / Gene Transfer / Interferon Alpha / Gene expression / Quantitative analysis / real time PCR / Humans / Female / Gene transfer techniques / Lentivirus / Quantitative Analysis / Transgene Expression / Gene Transfer / Interferon Alpha

Perilipin 2 Improves Insulin Sensitivity in Skeletal Muscle Despite Elevated Intramuscular Lipid Levels

Skeletal muscle biology / Diabetes / Membrane Proteins / RNA interference / Insulin Resistance / Cell line / Mice / Electroporation / Female / Animals / Gene transfer techniques / Male / Lipid metabolism / Rats / Oxidative phosphorylation / Wistar Rats / Recombinant Proteins / Gene expression profiling / Cell line / Mice / Electroporation / Female / Animals / Gene transfer techniques / Male / Lipid metabolism / Rats / Oxidative phosphorylation / Wistar Rats / Recombinant Proteins / Gene expression profiling

Lentiviral Vector Gene Transfer into Fetal Rhesus Monkeys (Macaca mulatta): Lung-Targeting Approaches

Genetics / Technology / Flow Cytometry / Fluorescence Microscopy / Immunohistochemistry / Ultrasound / HIV / Biological Sciences / Molecular / Hematopoietic Stem Cells / Humans / Animals / Blood sampling / Gene transfer techniques / Polymerase Chain Reaction / Lung / Rhesus Monkey / Model System / Macaca Mulatta / Transgene Expression / Molecular Targeted Therapy / Enzyme Linked Immunosorbent Assay / Gene Transfer / Ultrasound / HIV / Biological Sciences / Molecular / Hematopoietic Stem Cells / Humans / Animals / Blood sampling / Gene transfer techniques / Polymerase Chain Reaction / Lung / Rhesus Monkey / Model System / Macaca Mulatta / Transgene Expression / Molecular Targeted Therapy / Enzyme Linked Immunosorbent Assay / Gene Transfer

Apoptotic DNA endonuclease (DNase-gamma) gene transfer induces cell death accompanying DNA fragmentation in human glioma cells

Apoptosis / Western blotting / Cell Division / Neuro-Oncology / Humans / Glioma / Gene transfer techniques / Liposomes / Plasmids / Neurosciences / Brain Neoplasms / Glioma / Gene transfer techniques / Liposomes / Plasmids / Neurosciences / Brain Neoplasms

Carbonic anhydrase II promotes cardiomyocyte hypertrophy

Gene expression / Mice / Animals / Gene transfer techniques / Male / Medical Physiology / Rats / Phenylephrine / Heart Ventricles / Medical Physiology / Rats / Phenylephrine / Heart Ventricles

Accurate estimation of transduction efficiency necessitates a multiplex real-time PCR

Clinical Trial / Flow Cytometry / Gene Therapy / Gene expression / Biological Sciences / real time PCR / Humans / Mice / Computer Systems / Animals / Gene transfer techniques / Polymerase Chain Reaction / T cells / Sensitivity and Specificity / real time PCR / Humans / Mice / Computer Systems / Animals / Gene transfer techniques / Polymerase Chain Reaction / T cells / Sensitivity and Specificity

Transfer of a cathelicidin peptide antibiotic gene restores bacterial killing in a cystic fibrosis xenograft model

Cystic Fibrosis / Humans / Animals / Clinical / Gene transfer techniques / Staphylococcus aureus / Cell Transplantation / Pseudomonas aeruginosa / Anti-Bacterial Agents / Antimicrobial Cationic Peptides / Chickens / Swine / HeLa cells / Pseudomonas Aeruginosa / Clinical Investigation / Staphylococcus aureus / Cell Transplantation / Pseudomonas aeruginosa / Anti-Bacterial Agents / Antimicrobial Cationic Peptides / Chickens / Swine / HeLa cells / Pseudomonas Aeruginosa / Clinical Investigation

Bis-quaternary gemini surfactants as components of nonviral gene delivery systems: A comprehensive study from physicochemical properties to membrane interactions

Physical Chemistry / Pharmaceutics / DNA / Humans / Gene transfer techniques / Plasmids / HeLa cells / Surface Active Agents / Cell Survival / Molecular Structure / Plasmids / HeLa cells / Surface Active Agents / Cell Survival / Molecular Structure

Superoxide dismutase gene transfer reduces portal pressure in CCl4 cirrhotic rats with portal hypertension

Portal hypertension / Nitric oxide / Animals / Gene transfer techniques / Male / Vascular endothelium / Superoxide Dismutase / Clinical Sciences / Rats / Carbon Tetrachloride / Gut / Wistar Rats / Oxygen Consumption / Gene Transfer / Vascular endothelium / Superoxide Dismutase / Clinical Sciences / Rats / Carbon Tetrachloride / Gut / Wistar Rats / Oxygen Consumption / Gene Transfer

Mitochondrial leader sequence-plasmid DNA conjugates delivered into mammalian cells by DQAsomes co-localize with mitochondria

Genetics / Mitochondria / Confocal Microscopy / DNA / Humans / Gene transfer techniques / Peptides / Plasmids / Time Factors / Delivery System / Plasmid DNA / Oligonucleotides / Gene transfer techniques / Peptides / Plasmids / Time Factors / Delivery System / Plasmid DNA / Oligonucleotides

Adenovirus-mediated manganese superoxide dismutase gene transfer to hamster cheek pouch carcinoma cells

Cancer / Cell Adhesion / Gene expression / Cell Division / Humans / Animals / Gene transfer techniques / Superoxide Dismutase / Phenotype / Oral Squamous Cell Carcinoma (OSCC) / Cheek / Animals / Gene transfer techniques / Superoxide Dismutase / Phenotype / Oral Squamous Cell Carcinoma (OSCC) / Cheek

Efficient Non-Viral Ocular Gene Transfer with Compacted DNA Nanoparticles

Fluorescence Microscopy / Nanoparticles / Gene expression / Multidisciplinary / Recombinant DNA Technology / Humans / Mice / Animals / Gene transfer techniques / Retina / PLoS one / Eye / Transfection / Base Sequence / Recombinant Proteins / Drug Carriers / Gene Transfer / Humans / Mice / Animals / Gene transfer techniques / Retina / PLoS one / Eye / Transfection / Base Sequence / Recombinant Proteins / Drug Carriers / Gene Transfer
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